Li Biao, Tang Jia-wei, Cai Shao-qing, Li Xiao-mei
Department of Nephrology, Peking University First Hospital, Beijing 100034, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Aug 18;38(4):381-4.
To investigate whether Chinese herb astragalus and angelica mixture (A&A) have influence on the renal tubular epithelial cell injury induced by aristolochic-acid I.
Human proximal tubular epithelial cell line HK-2 was pre-treated with AA-I (2.5 mg/L) for 4 hours. Cells were then treated with or without A&A for additional 44 hours. Cell apoptosis was evaluated by using FACS. Secreted fibronectin (FN) and TGF-beta1 levels were assayed by ELISA. The changes of AA-I-induced FN, TGF-beta1 level and the rate of apoptosis were compared before and after A&A treatment.
There was basement secretion of TGF-beta1 by HK-2 cells (7.05+/-1.98 microg/L). Both normal serum (N-S) and A&A could not induce the cells to secret TGF-beta1(6.35+/-1.99 microg/L and 6.57+/-2.19 microg/L, vs. control, P>0.05). AA-I could induce the TGF-beta1 secretion by HK-2 cells (18.26+/-5.98 microg/L, vs. control, P<0.05). A&A could block AA-I-induced TGF-beta1 secretion by 63.5% (6.66+/-0.70 microg/L, vs. AA-I, P<0.05). It also suppressed AA-I-induced cell apoptosis by 93.7% (3.32%+/-0.41% vs. 19.19%+/-6.32% respectively, P<0.001) and FN secretion by 44% (1.64+/-1.11 folds vs. 2.93+/-0.87 folds respectively, P<0.05).
A&A inhibits AA-I induced injury in human renal proximal tubule epithelial cells, whose mechanism may be partially through blocking TGF-beta1 secretion.
研究中药黄芪当归合剂（A&A）对马兜铃酸I诱导的肾小管上皮细胞损伤是否有影响。
人近端肾小管上皮细胞系HK-2先用马兜铃酸I（2.5mg/L）预处理4小时。然后细胞再用或不用A&A处理44小时。采用流式细胞术评估细胞凋亡。用酶联免疫吸附测定法检测分泌型纤连蛋白（FN）和转化生长因子-β1（TGF-β1）水平。比较A&A处理前后马兜铃酸I诱导的FN、TGF-β1水平及凋亡率的变化。
HK-2细胞有基础水平的TGF-β1分泌（7.05±1.98μg/L）。正常血清（N-S）和A&A均不能诱导细胞分泌TGF-β1（分别为6.35±1.99μg/L和6.57±2.19μg/L，与对照组相比，P>0.05）。马兜铃酸I能诱导HK-2细胞分泌TGF-β1（18.26±5.98μg/L，与对照组相比，P<0.05）。A&A能使马兜铃酸I诱导的TGF-β1分泌减少63.5%（6.66±0.70μg/L，与马兜铃酸I组相比，P<0.05）。它还使马兜铃酸I诱导的细胞凋亡减少93.7%（分别为3.32%±0.41%和19.19%±6.32%，P<0.001），使FN分泌减少44%（分别为1.64±1.11倍和2.93±0.87倍，P<0.05）。
A&A抑制马兜铃酸I诱导的人肾近端小管上皮细胞损伤，其机制可能部分是通过阻断TGF-β1分泌实现的。
