Fan Bin, Liu Li-guo, Deng Xu-liang, Lu Hui-jun, Hu Xiao-yang, Wang Xin-zhi
Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Aug 18;38(4):428-31.
To prepare chicken egg yolk antibody (IgY) against Candida albicans and to study its biological properties in the interest of its further application.
Ten 25 weeks old laying hens were immunized with Candida albicans. IgY was isolated from the yolks of eggs by the water dilution (WD) method. Its yield, purity, and molecular weight were measured by Bradford method and Tris-tricine gel electrophoresis, respectively. The antigenicity of IgY was determined with Western blot method. The specific combination of IgY and Candida albicans was assayed with immunohistochemistry method. The activity of IgY was evaluated by enzyme-linked immunosorbent assay (ELISA).
The yield and purity of IgY were 7.9 g/L and 91.5%, respectively. It had the same molecular weight and antigenicity in comparison with IgY of chicken serum and normal chicken IgY. It could specifically combine with Candida albicans. Its titer was 1:12,000.
The production and purity of IgY were high. Its titer was good and did not decrease under a body temperature condition after 48 h. The IgY antibody had a combination capacity of specificity for Candida albicans in vitro.
制备抗白色念珠菌的鸡卵黄抗体（IgY）并研究其生物学特性，以便进一步应用。
用白色念珠菌对10只25周龄的产蛋母鸡进行免疫。采用水稀释（WD）法从鸡蛋蛋黄中分离IgY。分别用Bradford法和Tris-三羟甲基氨基甲烷凝胶电泳测定其产量、纯度和分子量。用蛋白质印迹法测定IgY的抗原性。用免疫组织化学方法检测IgY与白色念珠菌的特异性结合。通过酶联免疫吸附测定（ELISA）评估IgY的活性。
IgY的产量和纯度分别为7.9 g/L和91.5%。与鸡血清IgY和正常鸡IgY相比具有相同的分子量和抗原性。它能与白色念珠菌特异性结合。其效价为1:12,000。
IgY的产量和纯度较高。其效价良好，在体温条件下48小时后不降低。IgY抗体在体外对白色念珠菌具有特异性结合能力。
