Soni Pankaj, Banerjee Uttam Chand
Biocatalysis laboratory, Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, Nagar, India.
Biotechnol J. 2006 Jan;1(1):80-5. doi: 10.1002/biot.200500020.
The enantioselective bioreduction of acetophenone and its various analogues has been carried out using a new yeast strain, Candida tropicalis MTCC 5158, to obtain the corresponding (S)-aryl ethanols with good yield and almost absolute enantioselectivity. The catalytic ability of this microbial strain for acetophenone reduction has been examined and also various parameters of the bioreduction reaction have been optimized. Studies on the catalytic performance showed that this microorganism is capable of carrying out the reduction in a broad range of pH (3-10) and temperature (25-40 degrees C), making it a more versatile biocatalyst. The preparative scale bioreduction of acetophenone using resting cells of Candida tropicalis yielded S-(-)-1-phenyl ethanol with 43% yield and >99% enantiomeric excess.
使用新型酵母菌株热带假丝酵母MTCC 5158对苯乙酮及其各种类似物进行对映选择性生物还原，以高产率和几乎绝对的对映选择性获得相应的(S)-芳基乙醇。已研究了该微生物菌株对苯乙酮还原的催化能力，并优化了生物还原反应的各种参数。催化性能研究表明，这种微生物能够在广泛的pH值（3-10）和温度（25-40摄氏度）范围内进行还原反应，使其成为一种更通用的生物催化剂。使用热带假丝酵母的静息细胞对苯乙酮进行制备规模的生物还原，得到S-(-)-1-苯基乙醇，产率为43%，对映体过量>99%。
