泌乳期重组人乳铁蛋白转基因奶牛乳汁中的人乳铁蛋白和牛乳铁蛋白
Human and bovine lactoferrins in the milk of recombinant human lactoferrin-transgenic dairy cows during lactation.
作者信息
Hyvönen Paula, Suojala Leena, Haaranen Johanna, von Wright Atte, Pyörälä Satu
机构信息
Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland.
出版信息
Biotechnol J. 2006 Apr;1(4):410-2. doi: 10.1002/biot.200600016.
Seven Friesian human lactoferrin (hLf)-transgenic primiparous dairy cows expressing recombinant hLf (rhLf) in their milk were included in the study. After calving, concentrations of rhLf and bovine LF (bLf) in the milk, somatic cell count and milk yield were determined. The concentration of rhLf was found to be constant, about 2.9 mg/mL, throughout the early lactation period of 3 months. The concentration of bLf in colostrum was higher after calving, but decreased rapidly during the first days of lactation. The mean concentration of bLf was 0.15 mg/mL, but concentrations varied between cows from 0.07 mg/mL to 0.26 mg/mL. Based on that, it may be possible to improve the non-specific host defence mechanism in the mammary gland of dairy cows by enhancing the content of rhLf in the milk.
七头表达重组人乳铁蛋白（rhLf）的弗里斯兰人乳铁蛋白（hLf）转基因初产奶牛被纳入该研究。产犊后，测定了牛奶中rhLf和牛乳铁蛋白（bLf）的浓度、体细胞计数和产奶量。发现在整个3个月的早期泌乳期，rhLf的浓度是恒定的，约为2.9毫克/毫升。初乳中bLf的浓度在产犊后较高，但在泌乳的头几天迅速下降。bLf的平均浓度为0.15毫克/毫升，但不同奶牛之间的浓度在0.07毫克/毫升至0.26毫克/毫升之间变化。基于此，通过提高牛奶中rhLf的含量，有可能改善奶牛乳腺中的非特异性宿主防御机制。
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