Mouquet Hugo, Drouot Laurent, Charlionnet Roland, Arnoult Christophe, Bonnet-Bayeux Florence, Thomas Marlène, Leprince Jérôme, Joly Pascal, Tron François, Gilbert Danièle
Inserm, U519 and IFR23, Faculté de Médecine et de Pharmacie, Rouen, France.
Proteomics. 2006 Sep;6(17):4829-37. doi: 10.1002/pmic.200600257.
In most autoimmune diseases, the autoantibody response is directed against several antigens of the target organ whose identification is crucial for understanding the physiopathological process. Thus, technologies allowing a characterization of the whole autoantibody pattern of both human and experimental autoimmune diseases are required. Here we have used immunoproteomic analysis of human epidermal extracts to characterize the diversity of the anti-desmosome antibody response induced in normal mice immunized with desmoglein 1, the major autoantigen of pemphigus foliaceus, an autoimmune blistering skin disease. In particular, this analysis enables us to characterize the binding properties of anti-desmosome mAbs derived from these mice and to show that the autoantibody response induced upon immunization with a single autoantigen targets different epidermal autoantigens with a pattern similar to that observed in certain variety of human pemphigus.
在大多数自身免疫性疾病中，自身抗体反应针对的是靶器官的几种抗原，确定这些抗原对于理解生理病理过程至关重要。因此，需要能够对人类和实验性自身免疫性疾病的整个自身抗体模式进行表征的技术。在此，我们利用人表皮提取物的免疫蛋白质组学分析，来表征在用天疱疮落叶型（一种自身免疫性水疱性皮肤病）的主要自身抗原桥粒芯糖蛋白1免疫的正常小鼠中诱导产生的抗桥粒抗体反应的多样性。特别是，这种分析使我们能够表征源自这些小鼠的抗桥粒单克隆抗体的结合特性，并表明用单一自身抗原免疫后诱导的自身抗体反应以类似于在某些人类天疱疮中观察到的模式靶向不同的表皮自身抗原。
