非洲爪蟾水泥腺分化过程中的角蛋白基因差异表达。

Differential keratin gene expression during the differentiation of the cement gland of Xenopus laevis.

作者信息

LaFlamme S E, Dawid I B

机构信息

Laboratory of Molecular Genetics, National Institute of Child Health and Development, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Dev Biol. 1990 Feb;137(2):414-8. doi: 10.1016/0012-1606(90)90265-k.

DOI:10.1016/0012-1606(90)90265-k

PMID:1689262

Abstract

The expression of both epidermal and nonepidermal keratins has been detected in the cement gland of Xenopus laevis by antibody staining. Northern blot and in situ hybridizations with gene-specific probes indicated the expression of the nonepidermal keratin, XK endo B, and the embryonic epidermal keratin, XK70, in the cement gland. Furthermore, since explanted animal pole cells can be induced to differentiate into cement gland cells in vitro by incubation in NH4Cl, we have demonstrated the in vitro induction of XK endo B, maintenance of XK70, and repression of another embryonic epidermal keratin, XK81. This is the first report of keratin gene expression in the cement gland.

摘要

通过抗体染色在非洲爪蟾的黏液腺中检测到了表皮和非表皮角蛋白的表达。用基因特异性探针进行的Northern印迹和原位杂交表明，黏液腺中存在非表皮角蛋白XK endo B和胚胎表皮角蛋白XK70的表达。此外，由于将外植的动物极细胞置于氯化铵中培养可在体外诱导其分化为黏液腺细胞，我们已经证明了XK endo B在体外的诱导、XK70的维持以及另一种胚胎表皮角蛋白XK81的抑制。这是关于黏液腺中角蛋白基因表达的首次报道。