Breur-Vriesendorp B S, Vingerhoed J, Schaasberg W P, Ivanyi P
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Hum Immunol. 1990 Jan;27(1):1-15. doi: 10.1016/0198-8859(90)90091-3.
Allospecific anti-HLA class I antigen cytotoxic T-lymphocyte precursor frequencies (CTLpf) have been estimated in peripheral blood of healthy blood donors with responder stimulator combinations mismatched for one HLA-A,B antigen. The CTLpf ranged from 1 in 400 to 1 in 10,000, with most frequent values of 1 in 600 to 4000. The following observations were made: (1) CTLpf against the same HLA antigen vary among different responders; (2) CTLpf of one responder against various HLA antigens may be different; (3) "narrow" responders produce cytotoxic T lymphocytes that recognize only the private (stimulator) alloantigen, while "broad" responders produce mainly broadly cross-reactive cytotoxic T lymphocytes with public specificity. Split-well analysis shows that very few cytotoxic T lymphocytes of "broad" responders recognize the private alloantigen only. These individual variations are not dependent on the HLA phenotype, because they also occurred in unrelated HLA-identical responders stimulated against the same mismatched stimulator cells.
采用针对一种HLA - A、B抗原不相匹配的应答者 - 刺激细胞组合，对健康献血者外周血中同种特异性抗HLA - I类抗原细胞毒性T淋巴细胞前体频率（CTLpf）进行了评估。CTLpf范围为1/400至1/10,000，最常见的值为1/600至1/4000。得到以下观察结果：（1）不同应答者针对同一HLA抗原的CTLpf有所不同；（2）一名应答者针对各种HLA抗原的CTLpf可能不同；（3）“窄谱”应答者产生仅识别私有（刺激）同种抗原的细胞毒性T淋巴细胞，而“宽谱”应答者主要产生具有公共特异性的广泛交叉反应性细胞毒性T淋巴细胞。分孔分析表明，“宽谱”应答者中极少数细胞毒性T淋巴细胞仅识别私有同种抗原。这些个体差异不依赖于HLA表型，因为它们也出现在针对相同不相匹配刺激细胞进行刺激的不相关HLA相同应答者中。
