前列腺癌中SRC1基因的遗传和表达改变筛查。
Screening of genetic and expression alterations of SRC1 gene in prostate cancer.
作者信息
Mäki Hanna E, Waltering Kati K, Wallén Mika J, Martikainen Paula M, Tammela Teuvo L J, van Weerden Wytske M, Vessella Robert L, Visakorpi Tapio
机构信息
Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
出版信息
Prostate. 2006 Sep 15;66(13):1391-8. doi: 10.1002/pros.20427.
BACKGROUND
Genetic alterations of the SRC1 gene have not been thoroughly studied in prostate cancer.
MATERIALS AND METHODS
Five prostate cancer cell lines and 32 xenografts were screened for mutations and gene copy number alterations. Subsequently, frequencies of detected sequence variations were further analyzed in 44 clinical prostate cancers, 6 benign prostate hyperplasias, and 48 normal controls. Finally, the protein expression of SRC1 in 254 clinical prostate tumors was investigated.
RESULTS
Three non-recurrent sequence variations, and one single nucleotide polymorphism in the coding region of SRC1, as well as one case of SRC1 gene amplification were found. The protein expression of SRC1 was higher in androgen ablation resistant than untreated prostate carcinomas, but the difference was not statistically significant (P = 0.0796).
CONCLUSIONS
Genetic alterations of SRC1 are rare in prostate cancer. The nuclear protein accumulation of SRC1 seems to be mildly increased in androgen ablation resistant prostate cancers. .
背景
SRC1基因的遗传改变在前列腺癌中尚未得到充分研究。
材料与方法
对5种前列腺癌细胞系和32个异种移植瘤进行突变和基因拷贝数改变筛查。随后，在44例临床前列腺癌、6例良性前列腺增生和48例正常对照中进一步分析检测到的序列变异频率。最后，研究了254例临床前列腺肿瘤中SRC1的蛋白表达。
结果
在SRC1编码区发现3个非复发性序列变异、1个单核苷酸多态性以及1例SRC1基因扩增。SRC1的蛋白表达在雄激素消融抵抗的前列腺癌中高于未治疗的前列腺癌，但差异无统计学意义（P = 0.0796）。
结论
SRC1的遗传改变在前列腺癌中罕见。SRC1的核蛋白积累在雄激素消融抵抗的前列腺癌中似乎略有增加。
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