白色念珠菌，贝托，1923年：水解酶活性及消化道菌株生物分型的自有方法。

Candida albicans Berthout, 1923: hydrolases activity and own method of digestive tract strains biotyping.

作者信息

Kurnatowski Marek, Wasowska-Królikowska Krystyna, Kurnatowska Alicja

机构信息

Clinic of Children's Gastroenterology and Allergology, Medical University, Sporna 36/50, 91-738 Lódź.

出版信息

Wiad Parazytol. 2002;48(4):441-5.

PMID:16894729

Abstract

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMérieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e2 - phosphatase alcaline, e6 - leucine arylamidase, e11 - phosphatase acid, e5 - lipase (c14); e7 - valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase, e16 - alpha-glucosidase and e18 - N-acetyl-beta-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4 : 1): all "+"; one "-", three "+"; two "-", two "+"; three "-", one "+"; all "-". We have found the following biotypes: A (16.1 +/- 3.5%), B1 (2.7 +/- 2.53%), B3 (8.0 +/- 2.5%), B4 (22.3 +/- 3.9%), C2 (1.8 +/- 1.3%), C3 (7.1 +/- 2.4%), C6 (30.4 +/- 4.3%), D, (11.6 +/- 3.0%).

摘要

从患有胃肠道疾病的5至17岁儿童的口腔和上消化道群落（内窥镜检查）中分离出112株白色念珠菌。通过API 20C AUX和API ZYM测试（生物梅里埃公司）对无菌菌株进行鉴别。然后根据19种水解酶的活性确定所有菌株的酶谱和生物型。观察到以下酶活性最高：e2 - 碱性磷酸酶、e6 - 亮氨酸芳基酰胺酶、e11 - 酸性磷酸酶、e5 - 脂肪酶（c14）；e7 - 缬氨酸芳基酰胺酶、e12 - 萘酚 - AS - BI - 磷酸水解酶、e16 - α - 葡萄糖苷酶和e18 - N - 乙酰 - β - 葡萄糖苷酶，后四种用于生物分型程序。我们自己的系统基于数学二项分布公式（1 : 4 : 6 : 4 : 1）：全为“+”；一个“-”，三个“+”；两个“-”，两个“+”；三个“-”，一个“+”；全为“-”。我们发现了以下生物型：A（16.1 +/- 3.5%）、B1（2.7 +/- 2.53%）、B3（8.0 +/- 2.5%）、B4（22.3 +/- 3.9%）、C2（1.8 +/- 1.3%）、C3（7.1 +/- 2.4%）、C6（30.4 +/- 4.3%）、D（11.6 +/- 3.0%）。