[巢式聚合酶链反应在艾滋病患者卡氏肺孢子虫肺炎（PCP）诊断中的应用]

[Nested PCR in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients].

作者信息

Gołab E, Sobolewska A, Bitkowska E, Bednarska A, Berak H, Dzbeński T H, Horban A

机构信息

Zakład Parazytologii Lekarskiej, 00-791 Warszawa, ul. Chocimska 24.

出版信息

Wiad Parazytol. 2001;47(3):521-5.

PMID:16894769

Abstract

The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory tract were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.

摘要

开展这些研究是为了评估巢式聚合酶链反应（PCR）检测法在诊断艾滋病患者卡氏肺孢子虫肺炎（PCP）中的作用。为实现这一目的，从有呼吸道症状的HIV感染患者中收集了51份呼吸道排泄物样本，并检测其中特定DNA的存在情况。使用外部引物对pAZ 102E、pAZ 102H和内部引物pAZ 102X、pAZ 102Y扩增卡氏肺孢子虫线粒体大亚基rRNA基因的一部分。在51份检测样本中，有36份获得了阳性巢式PCR结果。约52.8%的阳性结果来自未临床诊断为PCP的患者所采集的样本。得出的结论是，巢式PCR方法过于敏感，不适用于艾滋病患者PCP的常规诊断。