Petrusevska Katerina, Kuznetsov Mikhail A, Gedicke Knut, Meshko Vera, Staroverov Sergey M, Seidel-Morgenstern Andreas
Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Republic of Macedonia.
J Sep Sci. 2006 Jul;29(10):1447-57. doi: 10.1002/jssc.200600036.
The separation of the enantiomers of several a-amino acids was studied on a new chiral stationary phase (CSP) which is based on the macrocyclic glycopeptide antibiotic eremomycin attached to silica particles. Retention and separation factors were determined under analytical conditions at ambient temperature for different mobile phase compositions. In order to evaluate the potential with respect to preparative separations the adsorption isotherms of D- and L-methionine were determined for one mobile phase composition applying the elution by characteristic point method. The isotherms were validated by comparing experimentally determined elution profiles with predictions based on the equilibrium dispersive model. Finally, the performance of the eremomycin CSP was compared with a commercially available CSP based on the macrocyclic antibiotic teicoplanin. After determining the isotherms of D- and L-methionine also for the teicoplanin phase, the equilibrium dispersive model was used for both CSP to identify optimal operating conditions. For the separation and conditions considered the new eremomycin CSP revealed a better performance compared to the teicoplanin CSP.
在一种基于连接到硅胶颗粒上的大环糖肽抗生素埃瑞霉素的新型手性固定相（CSP）上，研究了几种α-氨基酸对映体的分离。在不同流动相组成的环境温度分析条件下，测定了保留因子和分离因子。为了评估其在制备分离方面的潜力，采用特征点洗脱法测定了一种流动相组成下D-和L-蛋氨酸的吸附等温线。通过将实验测定的洗脱曲线与基于平衡分散模型的预测结果进行比较，对等温线进行了验证。最后，将埃瑞霉素CSP的性能与基于大环抗生素替考拉宁的市售CSP进行了比较。在测定了替考拉宁固定相的D-和L-蛋氨酸等温线后，利用平衡分散模型对两种CSP确定了最佳操作条件。在所考虑的分离和条件下，新型埃瑞霉素CSP显示出比替考拉宁CSP更好的性能。
