Staroverov S M, Kuznetsov M A, Nesterenko P N, Vasiarov G G, Katrukha G S, Fedorova G B
Chemical Department, Lomonosov State University, Leninskie Gory, 119992 Moscow, Russian Federation.
J Chromatogr A. 2006 Mar 10;1108(2):263-7. doi: 10.1016/j.chroma.2006.01.073. Epub 2006 Feb 7.
A new chiral stationary phase (CSP) was prepared by attachment of macrocyclic glycopeptide antibiotic eremomycin to the epoxy-activated silica under mild conditions. In contrast to CSP with immobilized vancomycin, which is a close structural analogue of eremomycin, the prepared CSP reveals high enantioselectivity for separation of amino acids enantiomers. It was demonstrated by the example of ristocetin A CSP that method of the immobilization of macrocyclic glycopeptide antibiotics affects remarkably the resulting enantioselectivity.
在温和条件下，通过将大环糖肽抗生素埃瑞霉素附着到环氧活化硅胶上制备了一种新型手性固定相（CSP）。与固定化万古霉素的CSP（万古霉素是埃瑞霉素的紧密结构类似物）相比，制备的CSP对氨基酸对映体的分离显示出高对映选择性。以瑞斯托菌素A CSP为例表明，大环糖肽抗生素的固定化方法对所得对映选择性有显著影响。
