Li Xia, Weng Hai-Bo, Han Shao-Yin, Xi Yu, Yong Ke-Lan
College of Life Sciences of Shanghai University, Shanghai 200436, China.
Sheng Wu Gong Cheng Xue Bao. 2006 Jul;22(4):550-4.
A novel practical binary vector to get marker-free transgenic plant was constructed. The estrogen-inducible Cre/loxP DNA recombination system was adopted in this system. All non-target genes located between two identical orientation loxP sites could be excised from the transgenic genome by the Cre expression. In order to analyze this system, the target gene, GUS expression box (CaMV35s: :GUS), was inserted in the MCS outside the region franked by two loxP sites. Then it was introduced into the tobaccos. Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation.
构建了一种用于获得无标记转基因植物的新型实用二元载体。该系统采用了雌激素诱导型Cre/loxP DNA重组系统。位于两个同向loxP位点之间的所有非靶基因可通过Cre表达从转基因基因组中切除。为了分析该系统，将靶基因GUS表达盒（CaMV35s::GUS）插入到由两个loxP位点界定区域之外的多克隆位点中。然后将其导入烟草。结果表明，发生了高效的DNA重组，且DNA激发后靶基因工作正常。
