[30%乙醇逆行注射大鼠输精管的抗生育作用]

[Antifertility effect of 30% ethanol retro-injection into rat vas deferens].

作者信息

Zhou Zhang-yan, Hu Li-quan, Wang Huai-peng, Li Shi-wen, Ma Sheng-li, Tang Qing

机构信息

Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.

出版信息

Zhonghua Nan Ke Xue. 2006 Jul;12(7):602-4.

PMID:16894935

Abstract

OBJECTIVE

To explore the antifertility effect and safety of 30% ethanol retro-injection into the vas deferens of the rat.

METHODS

Thirty Sprague-Dawley male rats, 3 m of age and (200 +/- 20) g in weight, were equally randomized into an experimental group and a control group. The former received 30% ethanol (0.5 ml) and the latter 0.9% sodium chloride (0.5 ml), both retro-injected into the vas deferens. Pregnancy rates were obtained through pregnancy tests with 60 Sprague-Dawley female adult rats 1.5 m and 3 m after the injection. All the male rats were sacrificed three months later, and tests were done for the rates of sperm motility and deformity as well as for the apoptosis of spermatogenic cells with TUNEL.

RESULTS

The 1.5 m pregnancy rate was 0 and the 3 m sperm motility and pregnancy rates were (0.32 +/- 1.12)% and (0.58 +/- 1.27)%, significantly decreased (P < 0.05) as compared with those of the control group, which were (80.62 +/- 2.68)%, (70.68 +/- 1.62)% and (86.62 +/- 1.68)%, respectively. While the 3 m sperm deformity rate in the experimental group was (78.26 +/- 1.08)%, increased significantly (P < 0.05), and the apoptosis index (AI) of spermatogenic cells was (7.63 +/- 1.16)% as compared with (5.62 +/- 1.32)% of the control group, with no significant difference between the two groups (P > 0.05).

CONCLUSION

Retro-injection of 30% ethanol into the vas deferens of the rat produces significant antifertility effect on rats, but has no significant influence on their spermatogenic cells.

摘要

目的

探讨30%乙醇逆行注射至大鼠输精管的抗生育作用及安全性。

方法

将30只3月龄、体重（200±20）g的Sprague-Dawley雄性大鼠随机分为实验组和对照组，每组各15只。实验组大鼠逆行注射30%乙醇（0.5 ml），对照组大鼠逆行注射0.9%氯化钠溶液（0.5 ml）。在注射后1.5个月和3个月，分别与60只成年Sprague-Dawley雌性大鼠交配，观察妊娠率。3个月后处死所有雄性大鼠，检测精子活力、畸形率，并采用TUNEL法检测生精细胞凋亡情况。

结果

注射后1.5个月的妊娠率为0；3个月时，实验组大鼠的精子活力率和妊娠率分别为（0.32±1.12）%和（0.58±1.27）%，与对照组[分别为（80.62±2.68）%、（70.68±1.62）%和（86.62±1.68）%]相比，均显著降低（P<0.05）。实验组大鼠3个月时的精子畸形率为（78.26±1.08）%，显著升高（P<0.05）；生精细胞凋亡指数（AI）为（7.63±1.16）%，与对照组的（5.62±1.32）%相比，差异无统计学意义（P>0.05）。