Song Hong-Xiu, Wang Ran, Geng Zhi-Ming, Cao Shao-Xian, Liu Tie-Zheng
Institute of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
Zhonghua Nan Ke Xue. 2008 May;14(5):406-10.
To explore the effect of subchronic exposure to acrylamide on the reproduction and testis endocrine function of rats.
Forty healthy adult male SD rats were randomly divided into 4 groups of equal number, exposed to acrylamide at the dose of 0, 4, 10 and 18 mg/(kg x d) respectively for 9 weeks, and then subjected to the determination of the hindlimb landing foot splay, sperm vitality and morphology, the activities of acid phosphatase (ACP) and alkaline phosphatase (ALP) in the testis homogenate, and the levels of testosterone (T) and estradiol (E2) in the serum and testis homogenate. Based on the primary Leydig cell culture models exposed to acrylamide of 0, 0.1, 0.75, 4 and 8 mmol/L, the activity of Leydig cells was measured by the CCK-8 method.
Following acrylamide exposure, the hindlimb landing foot splay increased markedly with dose increase (P < 0.01). The rates of sperm vitality were (6.86 +/- 5.46)%, (65.43 +/- 5.16)%, (60.86 +/- 4.26)% and (46.86 +/- 2.73)% in the exposed groups, significantly lower than in the control (P < 0.01); the rates of abnormal sperm were (39.00 +/- 10.95)%, (35.43 +/- 7.54)%, (45.71 +/- 13.28)% and (56.71 +/- 17.01)%, significantly increased in the 10 and 18 mg/(kg x d) groups (P < 0.05); ACP activities were (82.93 +/- 11.05), (73.52 +/- 8.77), (77.67 +/- 3.04) and (68.56 +/- 3.09) U/g prot, showing a decreasing tendency, while ALP activities were (0.96 +/- 0.15), (1.07 +/- 0.22), (1.12 +/- 0.22) and (0.74 +/- 0.10) U/g prot, displaying a tendency of first increasing and then decreasing. Both ACP and ALP activities were inhibited significantly in the 18 mg/(kg x d) group as compared with the control (P < 0.05). A marked reduction was noted in T levels in the serum, (13.44 +/- 4.76), (7.69 +/- 3.84), (5.23 +/- 1.42) and (1.36 +/- 0.86) ng/ml, as well as in the testis homogenate, (4.95 +/- 1.64), (3.01 +/- 0.76), (2.44 +/- 0.91) and (0.85 +/- 0.49) ng/mg prot, (P < 0.01), but no significant changes were observed in 17beta-E2 levels. After 24 hours exposure to acrylamide, the optical densities were 0.82 +/- 0.06, 0.56 +/- 0.07, 0.44 +/- 0.06, 0.26 +/- 0.03 and 0.45 +/- 0.21, showing an evident inhibition of the activity of Leydig cells at the dose of 0.1, 0.75, 4 and 8 mmol/L (P < 0.01).
Subchronic exposure to acrylamide could affect the normal development of sperm, cause changes of the activity of some enzymes in the testis and significantly influence hindlimb motor coordination. Acrylamide directly damages Leydig cells and affects the endocrine function of the testis.
探讨亚慢性丙烯酰胺暴露对大鼠生殖及睾丸内分泌功能的影响。
将40只健康成年雄性SD大鼠随机分为4组，每组数量相等，分别以0、4、10和18 mg/(kg·d)的剂量暴露于丙烯酰胺9周，然后测定后肢着地足展、精子活力和形态、睾丸匀浆中酸性磷酸酶（ACP）和碱性磷酸酶（ALP）的活性以及血清和睾丸匀浆中睾酮（T）和雌二醇（E2）的水平。基于暴露于0、0.1、0.75、4和8 mmol/L丙烯酰胺的原代睾丸间质细胞培养模型，采用CCK-8法测定睾丸间质细胞的活性。
丙烯酰胺暴露后，后肢着地足展随剂量增加而显著增加（P < 0.01）。暴露组精子活力率分别为(6.86 ± 5.46)%、(65.43 ± 5.16)%、(60.86 ± 4.26)%和(46.86 ± 2.73)%，显著低于对照组（P < 0.01）；异常精子率分别为(39.00 ± 10.95)%、(35.43 ± 7.54)%、(45.71 ± 13.28)%和(56.71 ± 17.01)%，10和18 mg/(kg·d)组显著升高（P < 0.05）；ACP活性分别为(82.93 ± 11.05)、(73.52 ± 8.77)、(77.67 ± 3.04)和(68.56 ± 3.09) U/g蛋白，呈下降趋势，而ALP活性分别为(0.96 ± 0.15)、(1.07 ± 0.22)、(1.12 ± 0.22)和(0.74 ± 0.10) U/g蛋白，呈先升高后降低的趋势。与对照组相比，18 mg/(kg·d)组ACP和ALP活性均受到显著抑制（P < 0.05）。血清中T水平显著降低，分别为(13.44 ± 4.76)、(7.69 ± 3.84)、(5.23 ± 1.42)和(1.36 ± 0.86) ng/ml，睾丸匀浆中T水平也显著降低，分别为(4.95 ± 1.64)、(3.01 ± 0.76)、(
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