流动注射化学发光法测定药物制剂和生物体液中的氧氟沙星和左氧氟沙星。

Flow-injection chemiluminescence determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids.

作者信息

Sun Hanwen, Li Liqing, Chen Xueyan

机构信息

College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Boading, China.

出版信息

Anal Sci. 2006 Aug;22(8):1145-9. doi: 10.2116/analsci.22.1145.

DOI:10.2116/analsci.22.1145

PMID:16896259

Abstract

A novel, rapid and sensitive analytical method is described for determination of ofloxacin and levofloxacin by enhanced chemiluminescence (CL) with flow-injection sampling. The method is based on the CL reaction of the Ce(IV)-Na2S2O4-ofloxacin/levofloxacin-H2SO2 system. The enhanced CL mechanism was developed and the optimum conditions for CL emission were investigated. The CL intensity was correlated linearly (r = 0.9988) with the concentration of ofloxacin (or levofloxacin) in the range of 1.0 x 10(-8) - 1.0 x 10(-7) g ml(-1) and 1.0 x 10(-7) - 6.0 x 10(-6) g ml(-1). The detection limit (S/N = 3) is 7 x 10(-9) g ml(-1). The relative standard derivation (RSD, n = 11) is 2.0% for ofloxacin at 4 x 10(-7) g ml(-1) and for levofloxacin at 6 x 10(-7) g ml(-1). This method has been successfully applied for the determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids with satisfactory results.

摘要

描述了一种用于通过流动注射采样增强化学发光（CL）法测定氧氟沙星和左氧氟沙星的新颖、快速且灵敏的分析方法。该方法基于Ce(IV)-Na2S2O4-氧氟沙星/左氧氟沙星-H2SO2体系的化学发光反应。研究了增强化学发光的机理并考察了化学发光发射的最佳条件。在1.0×10(-8)-1.0×10(-7) g ml(-1)和1.0×10(-7)-6.0×10(-6) g ml(-1)范围内，化学发光强度与氧氟沙星（或左氧氟沙星）浓度呈线性相关（r = 0.9988）。检测限（S/N = 3）为7×10(-9) g ml(-1)。在4×10(-7) g ml(-1)时，氧氟沙星的相对标准偏差（RSD，n = 11）为2.0%；在6×10(-7) g ml(-1)时，左氧氟沙星的相对标准偏差为2.0%。该方法已成功应用于药物制剂和生物流体中氧氟沙星和左氧氟沙星的测定，结果令人满意。