Jordi W, Nibbeling R, de Kruijff B
Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands.
FEBS Lett. 1990 Feb 12;261(1):55-8. doi: 10.1016/0014-5793(90)80635-v.
The interaction of phenethyl alcohol with model membranes and its effect on translocation of the chemically prepared mitochondrial precursor protein apocytochrome c across a lipid bilayer was studied. Phenethyl alcohol efficiently penetrates into monolayers and causes acyl chain disordering judged from deuterium nuclear magnetic resonance measurements with specific acyl chain-deuterated phospholipids. Translocation of apocytochrome c across a phospholipid bilayer was stimulated on addition of phenethyl alcohol indicating that the efficiency of translocation of this precursor protein is enhanced due to a disorder of the acyl chain region of the bilayer.
研究了苯乙醇与模型膜的相互作用及其对化学制备的线粒体前体蛋白脱辅基细胞色素c跨脂质双层转运的影响。苯乙醇能有效穿透单层膜，通过使用特定酰基链氘代磷脂的氘核磁共振测量判断，它会导致酰基链无序化。添加苯乙醇后，脱辅基细胞色素c跨磷脂双层的转运受到刺激，这表明由于双层膜酰基链区域的无序化，该前体蛋白的转运效率得到了提高。
