Corpeleijn E, Feskens E J M, Jansen E H J M, Mensink M, Saris W H M, de Bruin T W A, Blaak E E
Department of Human Biology, The Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands.
Diabetologia. 2006 Oct;49(10):2392-401. doi: 10.1007/s00125-006-0383-4. Epub 2006 Aug 3.
AIMS/HYPOTHESIS: The aim of this study was to investigate whether lifestyle intervention-induced changes in serum fatty acid profile of cholesteryl esters and estimated desaturase activities are related to improvements in insulin sensitivity in subjects at risk of type 2 diabetes.
In the Study on Lifestyle Intervention and Impaired Glucose Tolerance Maastricht (SLIM), 97 men and women with IGT were randomised to a combined diet and exercise programme (47 intervention) or a control group (50 control subjects). At baseline and after 1 year the following assessments were made: an OGTT, an exercise test to determine maximal aerobic capacity, anthropometry, and analysis of the serum fatty acid profile of cholesteryl esters.
The lifestyle programme was effective in reducing the intake of total and saturated fat, increasing physical activity, reducing obesity and improving insulin sensitivity and glucose tolerance. Regression analysis of the total population showed that an increase in the C20:4 n-6/C20:3 n-6 ratio (estimated Delta5-desaturase activity) and reductions in the C18:3 n-6/C18:2 n-6 ratio (estimated Delta6-desaturase activity) and the C16:1 n-7/C16:0 ratio (estimated Delta9-desaturase activity or stearoyl-CoA desaturase-1) were significantly associated with a decrease in homeostasis model assessment for insulin resistance. After adjustment for lifestyle changes (change in percentage body fat, aerobic capacity and saturated fat intake), these associations were partly reduced, but remained statistically significant.
CONCLUSIONS/INTERPRETATION: Lifestyle-induced changes in fatty acid profile of cholesteryl esters and desaturase activities were independently related to changes in insulin sensitivity in subjects at risk of type 2 diabetes.
目的/假设：本研究旨在调查生活方式干预引起的胆固醇酯血清脂肪酸谱变化和估计的去饱和酶活性是否与2型糖尿病风险受试者胰岛素敏感性的改善有关。
在马斯特里赫特生活方式干预与糖耐量受损研究（SLIM）中，97名糖耐量受损的男性和女性被随机分为联合饮食和运动计划组（47名干预组）或对照组（50名对照受试者）。在基线和1年后进行了以下评估：口服葡萄糖耐量试验、测定最大有氧能力的运动试验、人体测量以及胆固醇酯血清脂肪酸谱分析。
生活方式计划有效地减少了总脂肪和饱和脂肪的摄入量，增加了体力活动，减轻了肥胖，并改善了胰岛素敏感性和葡萄糖耐量。对总体人群的回归分析表明，C20:4 n-6/C20:3 n-6比值（估计的Δ⁵-去饱和酶活性）增加，C18:3 n-6/C18:2 n-6比值（估计的Δ⁶-去饱和酶活性）和C16:1 n-7/C16:0比值（估计的Δ⁹-去饱和酶活性或硬脂酰辅酶A去饱和酶-1）降低与胰岛素抵抗稳态模型评估的降低显著相关。在调整生活方式变化（体脂百分比变化、有氧能力和饱和脂肪摄入量）后；这些关联部分减弱，但仍具有统计学意义。
结论/解读：生活方式引起的胆固醇酯脂肪酸谱和去饱和酶活性变化与2型糖尿病风险受试者胰岛素敏感性变化独立相关。
