Wohlers Marta, Xavier Roberta Araujo Navarro, Oyama Lila Missae, Ribeiro Eliane Beraldi, do Nascimento Cláudia Maria Oller, Casarini Dulce Elena, Silveira Vera Lucia Flor
Physiology Department, Federal University of São Paulo-EPM, Rua Botucatu 862-2 andar, Vila Clementino-CEP, São Paulo 04023-060, Brazil.
Inflammation. 2005 Apr;29(2-3):81-9. doi: 10.1007/s10753-006-9002-2.
We have previously demonstrated that both n-3 and n-6 polyunsaturated fatty acids (PUFA)-rich diets decrease the acute inflammatory response partially explained by the high corticosterone basal levels. The present study aimed to determine the effect of hyperlipidic diets (PUFA n-3 or n-6) on phagocytosis, hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) release by macrophages, bradykinin (BK) and NO release in the paw inflammatory perfusate and Kallikrein (KK), corticosterone and leptin blood levels. Hyperlipidic diets decreased H(2)O(2) release from macrophages stimulated by carrageenan or phorbol-miristate-acetate (PMA), NO release from macrophage stimulated by carrageenan, BK and NO release in the edema perfusate, KK plasma levels and the increase of serum leptin after carrageenan stimulus. These data show that both fish and soybean oil-rich diets promote similar alterations on inflammatory mediators of carrageenan edema and a causal association with the anti-inflammatory effect of these diets.
我们之前已经证明，富含n-3和n-6多不饱和脂肪酸（PUFA）的饮食可降低急性炎症反应，这部分可由高皮质酮基础水平来解释。本研究旨在确定高脂饮食（n-3或n-6 PUFA）对巨噬细胞吞噬作用、过氧化氢（H₂O₂）和一氧化氮（NO）释放、爪部炎症灌流液中缓激肽（BK）和NO释放以及激肽释放酶（KK）、皮质酮和瘦素血液水平的影响。高脂饮食可降低角叉菜胶或佛波酯-肉豆蔻酸酯-乙酸酯（PMA）刺激的巨噬细胞释放H₂O₂、角叉菜胶刺激的巨噬细胞释放NO、水肿灌流液中的BK和NO释放、KK血浆水平以及角叉菜胶刺激后血清瘦素的增加。这些数据表明，富含鱼油和大豆油的饮食对角叉菜胶水肿的炎症介质产生相似的改变，并且与这些饮食的抗炎作用存在因果关系。
