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秀丽隐杆线虫的MAI-1蛋白与线粒体ATP酶抑制剂（IF1）相似，能够抑制酵母F0F1-ATP酶，但无法转运至酵母线粒体。

Caenorhabditis elegans MAI-1 protein, which is similar to mitochondrial ATPase inhibitor (IF1), can inhibit yeast F0F1-ATPase but cannot be transported to yeast mitochondria.

作者信息

Ichikawa Naoki, Ando Chiyoko, Fumino Mina

机构信息

Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka, 558-8585, Japan.

出版信息

J Bioenerg Biomembr. 2006 Apr;38(2):93-9. doi: 10.1007/s10863-006-9009-2. Epub 2006 Aug 2.

DOI:10.1007/s10863-006-9009-2
PMID:16897438
Abstract

In Caenorhabditis elegans, two proteins that are similar to mitochondrial ATPase inhibitor protein (IF(1)) have been found and named MAI-1 and MAI-2. In this study, we overexpressed and purified both the proteins and examined their properties. Circular dichroism spectra indicated that both the MAI-1 and MAI-2 predominantly consisted of beta- and random structure, and in contrast to mammalian IF(1), alpha-helixes were barely detected. Both MAI-1 and MAI-2 could inhibit yeast F(0)F(1)-ATPase, but the inhibition by MAI-1 was pH-independent. MAI-2-GFP fusion protein was transported to yeast mitochondria, but MAI-1-GFP was not. These results indicate that the MAI-2 is (C. elegans) IF(1). MAI-1 seems to be a cytosolic protein and may regulate cytosolic ATPase(s).

摘要

在秀丽隐杆线虫中，已发现两种与线粒体ATP酶抑制蛋白（IF(1)）相似的蛋白质，并将其命名为MAI-1和MAI-2。在本研究中，我们对这两种蛋白质进行了过表达和纯化，并检测了它们的特性。圆二色光谱表明，MAI-1和MAI-2主要由β-结构和无规结构组成，与哺乳动物IF(1)不同，几乎检测不到α-螺旋。MAI-1和MAI-2都能抑制酵母F(0)F(1)-ATP酶，但MAI-1的抑制作用不依赖于pH值。MAI-2-GFP融合蛋白被转运到酵母线粒体中，而MAI-1-GFP则没有。这些结果表明，MAI-2是（秀丽隐杆线虫的）IF(1)。MAI-1似乎是一种胞质蛋白，可能调节胞质ATP酶。

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2
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J Biochem. 2005 Aug;138(2):201-7. doi: 10.1093/jb/mvi116.
3
The binding mechanism of the yeast F1-ATPase inhibitory peptide: role of catalytic intermediates and enzyme turnover.
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Dev Cell. 2022 Feb 7;57(3):361-372.e5. doi: 10.1016/j.devcel.2021.12.016. Epub 2022 Jan 18.
4
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6
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