Myint Khin S, Endy Timothy P, Mongkolsirichaikul Duangrat, Manomuth Choompun, Kalayanarooj Siripen, Vaughn David W, Nisalak Ananda, Green Sharone, Rothman Alan L, Ennis Francis A, Libraty Daniel H
Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
J Infect Dis. 2006 Sep 1;194(5):600-7. doi: 10.1086/506451. Epub 2006 Jul 31.
Apoptosis is an important modulator of cellular immune responses during systemic viral infections. Peripheral-blood mononuclear cell (PBMC) apoptosis and plasma soluble levels of CD95, a mediator of apoptosis, were determined in sequential samples from children participating in a prospective study of dengue virus (DV) infections. During the period of defervescence, levels of PBMC apoptosis were higher in children developing dengue hemorrhagic fever (DHF), the most severe form of illness, than in those with dengue fever (DF) and other, nondengue, febrile illnesses. CD8(+) T lymphocytes made up approximately half of the peak circulating apoptotic PBMCs in DHF and DF. Maximum plasma levels of soluble CD95 were also higher in children with DHF than in those with DF. The level of PBMC apoptosis correlated with dengue disease severity. Apoptosis appears to be involved in modulation of the innate and adaptive immune responses to DV infection and is likely involved in the evolution of immune responses in other viral hemorrhagic fevers.
细胞凋亡是全身性病毒感染期间细胞免疫反应的重要调节因子。在参与登革病毒（DV）感染前瞻性研究的儿童的连续样本中，测定了外周血单核细胞（PBMC）凋亡情况以及凋亡介质CD95的血浆可溶性水平。在退热期，患登革出血热（DHF，最严重的疾病形式）的儿童的PBMC凋亡水平高于患登革热（DF）和其他非登革热发热性疾病的儿童。在DHF和DF中，CD8(+) T淋巴细胞约占循环凋亡PBMC峰值的一半。DHF患儿的可溶性CD95血浆最高水平也高于DF患儿。PBMC凋亡水平与登革热疾病严重程度相关。细胞凋亡似乎参与了对DV感染的固有免疫和适应性免疫反应的调节，并且可能参与了其他病毒性出血热免疫反应的演变。
