从土壤中分离出的真菌对乙酰氨基酚葡糖苷共轭物的形成

Formation of glucoside conjugate of acetaminophen by fungi separated from soil.

作者信息

Huang H H, Lin L H, Zhang P, Qi X L, Zhong D F

机构信息

Shanghai Drug-Metab Biotech Co., Ltd, People's Republic of China.

出版信息

Eur J Drug Metab Pharmacokinet. 2006 Apr-Jun;31(2):103-8. doi: 10.1007/BF03191126.

DOI:10.1007/BF03191126

PMID:16898078

Abstract

The phase II metabolite of acetaminophen in filamentous fungi and actinomycetes separated from soil was investigated. Fifty-four filamentous fungi and twenty-seven actinomycetes were screened to transform acetaminophen. The metabolites of acetaminophen were assayed using liquid chromatography-tandem mass spectrometry. The only metabolite was subject to enzymatic hydrolysis to confirm its structure. Acetaminophen was converted into glucoside conjugate, by filamentous fungi JX1-60, LN17-2, LN20-1 and the yield of the conjugate was 60.01%, 44.27%, 100%, respectively, and no phase I metabolites were detected. Glucoside conjugation of acetaminophen in filamentous fungi differs from the phase II metabolism of glucuronidation in humans. The fungus LN20-1 could be a suitable model to synthesize glucoside conjugate of acetaminophen.

摘要

对从土壤中分离出的丝状真菌和放线菌中对乙酰氨基酚的II期代谢产物进行了研究。筛选了54株丝状真菌和27株放线菌以转化对乙酰氨基酚。使用液相色谱-串联质谱法测定对乙酰氨基酚的代谢产物。唯一的代谢产物经过酶促水解以确认其结构。丝状真菌JX1-60、LN17-2、LN20-1将对乙酰氨基酚转化为葡糖苷缀合物，其缀合物的产率分别为60.01%、44.27%、100%，未检测到I期代谢产物。丝状真菌中对乙酰氨基酚的葡糖苷缀合不同于人类的葡糖醛酸化II期代谢。真菌LN20-1可能是合成对乙酰氨基酚葡糖苷缀合物的合适模型。