鸟嘌呤核苷酸调节P物质对大鼠纹状体腺苷酸环化酶的作用。

Guanine nucleotides regulate the effect of substance P on striatal adenylate cyclase of the rat.

作者信息

Moser A

机构信息

Dept. of Neurology, Med. University, Lübeck, F.R.G.

出版信息

Biochem Biophys Res Commun. 1990 Feb 28;167(1):211-5. doi: 10.1016/0006-291x(90)91752-e.

DOI:10.1016/0006-291x(90)91752-e

PMID:1690002

Abstract

Substance P was incubated in an adenylate cyclase assay of a particulate fraction of caudate-putamen tissue of the rat in order to examine the effect of the peptide on D-1 receptor coupled adenylate cyclase in vitro. Substance P did not influence basal adenylate cyclase activity or the stimulation of the enzyme by dopamine. No influence of substance P was seen on the effects of calcium and magnesium chloride as a cofactor of adenylate cyclase. Also the inhibition of adenylate cyclase activity by the dopamine antagonist fluphenazine was not influenced by substance P. However, substance P was able to enhance cyclic AMP formation in the presence of guanosine-imidodiphosphate (Gpp(NH)p), whereas the stimulatory effect of guanosine-triphosphate (GTP) was inhibited by substance P. In our study we suggest that substance P interacts with the guanine nucleotide regulatory subunit without directly affecting D-1 dopamine receptors in the caudate-putamen of the rat.

摘要

为了在体外检测P物质对与D-1受体偶联的腺苷酸环化酶的作用，将P物质与大鼠尾状核-壳核组织的微粒体部分一起进行腺苷酸环化酶测定。P物质不影响基础腺苷酸环化酶活性或多巴胺对该酶的刺激作用。未观察到P物质对作为腺苷酸环化酶辅因子的氯化钙和氯化镁的作用有影响。多巴胺拮抗剂氟奋乃静对腺苷酸环化酶活性的抑制作用也不受P物质影响。然而，在存在鸟苷亚氨二磷酸（Gpp(NH)p）的情况下，P物质能够增强环磷酸腺苷（cAMP）的形成，而三磷酸鸟苷（GTP）的刺激作用则被P物质抑制。在我们的研究中，我们认为P物质与鸟嘌呤核苷酸调节亚基相互作用，而不直接影响大鼠尾状核-壳核中的D-1多巴胺受体。