一种基于逆转录病毒的系统，通过RNA干扰稳定沉默乙型肝炎病毒基因。
A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference.
作者信息
Jia Fang, Zhang Yi-Zheng, Liu Chang-Mei
机构信息
School of Life Sciences, Sichuan University, Chengdu , 610064, China.
出版信息
Biotechnol Lett. 2006 Oct;28(20):1679-85. doi: 10.1007/s10529-006-9138-z. Epub 2006 Aug 10.
RNA interference (RNAi) might be an efficient antiviral therapy for some obstinate illness. Herein, a retrovirus-based RNAi system was developed to drive expression and delivery of Hepatitis B virus (HBV)-specific short hairpin RNA (shRNA) in HepG2 cells. The levels of HBsAg and HBeAg and that of HBV mRNA were dramatically decreased by this RNAi system in HepG2 cells transfected with Topo-HBV plasmid. Retrovirus-based RNAi thus may be useful for therapy in HBV and other viral infections and provide new clues for prophylactic vaccine development.
RNA干扰（RNAi）可能是治疗某些顽固性疾病的一种有效抗病毒疗法。在此，开发了一种基于逆转录病毒的RNAi系统，以驱动乙型肝炎病毒（HBV）特异性短发夹RNA（shRNA）在HepG2细胞中的表达和递送。在转染了Topo-HBV质粒的HepG2细胞中，该RNAi系统显著降低了HBsAg、HBeAg的水平以及HBV mRNA的水平。因此，基于逆转录病毒的RNAi可能对HBV及其他病毒感染的治疗有用，并为预防性疫苗的开发提供新线索。
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