Cystatin C in cerebrospinal fluid and multiple sclerosis.

作者信息

Hansson Sara F, Simonsen Anja Hviid, Zetterberg Henrik, Andersen Oluf, Haghighi Sara, Fagerberg Inger, Andréasson Ulf, Westman-Brinkmalm Ann, Wallin Anders, Rüetschi Ulla, Blennow Kaj

机构信息

Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden.

出版信息

Ann Neurol. 2007 Aug;62(2):193-6; discussion 205. doi: 10.1002/ana.20945.

OBJECTIVE

A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5 kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS).

METHODS

Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects.

RESULTS

Full-length CysC (13.4 kDa) concentration was similar in MS and control samples. The 12.5 kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at -20 degrees C.

INTERPRETATION

The 12.5 kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS.