Sadakata Tetsushi, Furuichi Teiichi
Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Neurosci Lett. 2006 Sep 25;405(3):217-22. doi: 10.1016/j.neulet.2006.07.008. Epub 2006 Aug 9.
By fluorescent differential display, we identified six transcripts (CAPS2/Cadps2, Cdh22, b1402, c1502, d1401, and d1501) that showed the differential expression patterns during the postnatal development of the mouse cerebellum. We further analyzed the latter four transcripts whose cellular localizations in developing mouse brains have not been studied. In the postnatal cerebellum, clones c1502 and d1501 were transiently up-regulated; clone b1402 was up-regulated; and clone d1401 remained relatively constant. Sequence analysis revealed that d1401 and c1502 were derivatives of Ogdh (oxoglutarate dehydrogenase) and QP-C (ubiquinol-cytochrome c reductase complex ubiquinone-binding protein), respectively. Moreover, b1402 and d1501 were identified as transcripts of a predicted gene (4933409K07Rik) and a novel EST, respectively. b1402 and d1501 were abundantly present in the cerebellum, whereas c1502/QP-C and d1401/Ogdh were widely distributed in various mouse tissues. In the postnatal mouse brain, moderately high mRNA levels of b1402 were restricted to the olfactory bulb, striatum, cerebral cortex (layers II-III and VI), hippocampus (dentate granule cells), and cerebellum (granule cells). c1502/QP-C mRNA was localized at high levels in the olfactory bulb, cerebral cortex, hippocampus, thalamus (anterodorsal nucleus, parafacicularis nucleus), tegmentum (red nucleus), cerebellum (Purkinje and granule cells), and pons (pontine nucleus, reticulotegmental nucleus, trapezoid body, vestibular nucleus). High mRNA levels of d1401/Ogdh were observed in the olfactory bulb, hippocampus, cerebellum, and pons, whereas those of d1501 were detected in the granule cells of the olfactory bulb, dentate gyrus, and cerebellum.
通过荧光差异显示技术，我们鉴定出六个转录本（CAPS2/Cadps2、Cdh22、b1402、c1502、d1401和d1501），它们在小鼠小脑出生后的发育过程中呈现出差异表达模式。我们进一步分析了后四个转录本，其在发育中小鼠大脑中的细胞定位尚未得到研究。在出生后的小脑中，克隆c1502和d1501瞬时上调；克隆b1402上调；克隆d1401保持相对稳定。序列分析表明，d1401和c1502分别是Ogdh（酮戊二酸脱氢酶）和QP-C（泛醌-细胞色素c还原酶复合物泛醌结合蛋白）的衍生物。此外，b1402和d1501分别被鉴定为一个预测基因（4933409K07Rik）和一个新EST的转录本。b1402和d1501在小脑中大量存在，而c1502/QP-C和d1401/Ogdh广泛分布于小鼠的各种组织中。在出生后的小鼠大脑中，b1402的中等偏高mRNA水平局限于嗅球、纹状体、大脑皮层（II-III层和VI层）、海马体（齿状颗粒细胞）和小脑（颗粒细胞）。c1502/QP-C mRNA在嗅球、大脑皮层、海马体、丘脑（前背核、束旁核）、被盖（红核）、小脑（浦肯野细胞和颗粒细胞）和脑桥（脑桥核、网状被盖核、斜方体、前庭核）中高水平定位。在嗅球、海马体、小脑和脑桥中观察到d1401/Ogdh的高mRNA水平，而在嗅球、齿状回和小脑的颗粒细胞中检测到d1501的mRNA水平。
