Ngotho Maina, Maina Naomi, Kagira John, Royo Felix, Farah Idle O, Hau Jann
KARI-Trypanosomiasis Research Centre, Kikuyu, Kenya.
Parasitol Int. 2006 Dec;55(4):243-8. doi: 10.1016/j.parint.2006.06.004. Epub 2006 Aug 8.
IL-10 has been suggested as a possible parameter for human African trypanosomiasis stage determination. However, conclusive experimental studies have not been carried out to evaluate this, which is a prerequisite before a potential test can be validated in humans for diagnostic purposes. We used the vervet monkey model of trypanosomiasis to scrutinize IL-10 in blood and cerebrospinal fluid (CSF). Five adult males were experimentally infected with T. b. rhodesiense. The infected animals became anemic and exhibited weight loss. Parasitemia was patent after 3 days and fluctuated around 3.7 x 10(7) trypanosomes/ml throughout the experimental period. The total CSF white cell counts increased from pre-infection means around 3 cells/micro l to a peak of 30 cells/micro l, 42 days post-infection (DPI). IL-10 was not detectable (<2 pg/ml) in serum prior to infection. IL-10 serum concentrations increased to 273 pg/ml 10 DPI coinciding with the first peak of parasitemia. Thereafter the levels declined to a mean value of 77 pg/ml 34 DPI followed by a significant rise to a second peak of 304 pg/ml (p<0.008) 42 DPI. There was no detectable IL-10 in CSF. IL-10 synthesis is thus stimulated both in the early and transitional stages of experimental trypanosomiasis. That IL-10 is produced in early stage disease is an interesting finding unlikely to be detected in humans where it is difficult to determine the exact time of infection. The IL-10 peak observed on day 42 of infection might indicate onset of parasite neuroinvasion coinciding with a peak in white blood cell counts in the blood and CSF.
白细胞介素-10已被提议作为人类非洲锥虫病分期的一个可能参数。然而，尚未进行确凿的实验研究来评估这一点，而这是在人体中验证一项潜在检测用于诊断目的之前的一个先决条件。我们使用锥虫病的黑长尾猴模型来仔细研究血液和脑脊液（CSF）中的白细胞介素-10。五只成年雄性黑长尾猴被实验性感染罗德西亚布氏锥虫。被感染的动物出现贫血并体重减轻。感染3天后出现虫血症，在整个实验期间虫血症水平在约3.7×10⁷个锥虫/毫升上下波动。脑脊液白细胞总数从感染前的平均约3个细胞/微升增加到感染后42天（DPI）的峰值30个细胞/微升。感染前血清中未检测到白细胞介素-10（<2 pg/ml）。白细胞介素-10血清浓度在感染后10天增加到273 pg/ml，与虫血症的第一个峰值同时出现。此后，水平下降到感染后34天的平均值77 pg/ml，随后在感染后42天显著上升到第二个峰值304 pg/ml（p<0.008）。脑脊液中未检测到白细胞介素-10。因此，在实验性锥虫病的早期和过渡阶段，白细胞介素-10的合成均受到刺激。白细胞介素-10在疾病早期产生是一个有趣的发现，在人类中不太可能检测到，因为在人类中很难确定确切的感染时间。感染后第42天观察到的白细胞介素-10峰值可能表明寄生虫神经侵袭的开始，与血液和脑脊液中白细胞计数的峰值同时出现。
