Yang Sheau-Fang, Wang Shen-Nien, Wu Chih-Fung, Yeh Yao-Tsung, Chai Chee-Yin, Chunag Shih-Chang, Sheen Maw-Chang, Lee King-Teh
Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
J Clin Pathol. 2007 Jun;60(6):642-8. doi: 10.1136/jcp.2006.036970. Epub 2006 Aug 10.
Constitutive activation of signal transducer and activator of transcription 3 at tyrosine residue 705 (p-STAT3 (tyr705)) has been associated with many types of human cancers. However, its potential roles and biological effects in hepatocellular carcinoma (HCC) are not well established.
To explore whether an altered p-STAT3 (tyr705) expression is associated with angiogenesis or proliferation and thereby plays a part in HCC development.
Paraffin-wax-embedded sections from 69 patients with HCC were collected in this study. Using a semiquantitative immunohistochemical staining method, the expression patterns of p-STAT3 (tyr705) in both HCC lesions and the adjacent non-tumorous liver parenchyma were analysed. The results obtained were further correlated with intratumour microvessel density (MVD), Ki-67 expression, clinicopathological parameters and overall survival.
A strong p-STAT3 (tyr705) nuclear staining was observed in 49.3% of HCC lesions, but was reported only in 5.8% of the adjacent non-tumorous liver parenchyma (p<0.001). The expression of p-STAT3 (tyr705) in HCC lesions was significantly and positively correlated with the intratumour MVD (p = 0.002), but not with Ki-67 expression. No significant correlation of p-STAT3 (tyr705) was found in addition to histological grading (p = 0.019). Multivariate Cox regression analysis showed that p-STAT3 (tyr705) expression was a significant predictor of overall survival for HCC (p = 0.036), although the Kaplan-Meier survival curves showed no significant difference between the high and low p-STAT3 (tyr705) expression subgroups.
The results showed that p-STAT3 (tyr705) expression was closely correlated with histological grading and intratumour MVD in HCC. Thus, the potential role of p-STAT3 (tyr705) in HCC development may be through these correlations.
信号转导子和转录激活子3在酪氨酸705位点的组成性激活（p-STAT3 (tyr705)）与多种人类癌症相关。然而，其在肝细胞癌（HCC）中的潜在作用和生物学效应尚未完全明确。
探讨p-STAT3 (tyr705)表达改变是否与血管生成或增殖相关，从而在HCC发生发展中发挥作用。
本研究收集了69例HCC患者的石蜡包埋切片。采用半定量免疫组织化学染色方法，分析p-STAT3 (tyr705)在HCC病灶及相邻非肿瘤性肝实质中的表达模式。所得结果进一步与肿瘤内微血管密度（MVD）、Ki-67表达、临床病理参数及总生存期相关联。
在49.3%的HCC病灶中观察到p-STAT3 (tyr705)强核染色，但在仅5.8%的相邻非肿瘤性肝实质中出现（p<0.001）。HCC病灶中p-STAT3 (tyr705)的表达与肿瘤内MVD显著正相关（p = 0.002），但与Ki-67表达无关。除组织学分级外，未发现p-STAT3 (tyr705)有显著相关性（p = 0.019）。多因素Cox回归分析显示，p-STAT3 (tyr705)表达是HCC总生存期的显著预测指标（p = 0.036），尽管Kaplan-Meier生存曲线显示高p-STAT3 (tyr705)表达亚组和低p-STAT3 (tyr705)表达亚组之间无显著差异。
结果表明，p-STAT3 (tyr705)表达与HCC的组织学分级和肿瘤内MVD密切相关。因此，p-STAT3 (tyr705)在HCC发生发展中的潜在作用可能通过这些相关性来实现。
