Grant A L, Thomas J W, King K J, Liesman J S
Dept. of Anim. Sci., Michigan State University, East Lansing 48824.
J Anim Sci. 1990 Feb;68(2):363-71. doi: 10.2527/1990.682363x.
Six litters of newborn crossbred piglets were utilized to examine 1) the effects of substituting 20% of the protein of an all-milk protein liquid diet with a soy protein isolate (milk-soy diet) on small intestinal variables and 2) the effects of supplementing this milk-soy diet with 25 g of either putrescine or ethylamine per kilogram diet on small intestinal variables. Small intestinal xylose absorption tended to increase from wk 1 to wk 2 of age in pigs fed the milk, putrescine and ethylamine diets, but not in pigs fed the milk, putrescine and ethylamine diets, but not in pigs fed the unsupplemented milk-soy diet. Crypt depth in pigs fed the milk-soy diet tended to be less (9.4%; P greater than .10) than the crypt depth in pigs fed the other diets, but mitotic index was not different (P greater than .10) among diets. Mucosal protein, DNA and RNA concentrations and mucosal brush border sucrase and cytosolic dipeptidase activities tended to be least in pigs fed the putrescine and ethylamine diets. Concentration of mucosal putrescine was greatest (P less than .002) in the distal regions of the small intestine of pigs fed putrescine. Mucosal ornithine decarboxylase activity was inhibited by putrescine (P less than .02), but it was not affected by the soybean protein isolate used in this study. Supplementing soy protein isolate diets with amines may enhance intestinal absorption and enterocyte proliferation.
1）用大豆分离蛋白替代全乳蛋白流质日粮中20%的蛋白质（乳 - 大豆日粮）对小肠变量的影响；2）在这种乳 - 大豆日粮中每千克日粮添加25克腐胺或乙胺对小肠变量的影响。在饲喂牛奶、腐胺和乙胺日粮的仔猪中，小肠木糖吸收量在1周龄到2周龄期间有增加趋势，但在饲喂未添加的乳 - 大豆日粮仔猪中没有这种趋势。饲喂乳 - 大豆日粮的仔猪隐窝深度比饲喂其他日粮的仔猪隐窝深度有降低趋势（9.4%；P大于0.10），但各日粮间有丝分裂指数没有差异（P大于0.10）。饲喂腐胺和乙胺日粮的仔猪黏膜蛋白、DNA和RNA浓度以及黏膜刷状缘蔗糖酶和胞质二肽酶活性往往最低。在饲喂腐胺的仔猪小肠远端区域，黏膜腐胺浓度最高（P小于0.002）。腐胺抑制黏膜鸟氨酸脱羧酶活性（P小于0.02），但本研究中使用的大豆分离蛋白对其没有影响。在大豆分离蛋白日粮中添加胺类可能会增强肠道吸收和肠上皮细胞增殖。
