Kondo Tatsuhiko, Sawa Shinichiro, Kinoshita Atsuko, Mizuno Satoko, Kakimoto Tatsuo, Fukuda Hiroo, Sakagami Youji
Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
Science. 2006 Aug 11;313(5788):845-8. doi: 10.1126/science.1128439.
The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell-specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12-amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.
拟南芥CLAVATA3（CLV3）基因编码一种干细胞特异性蛋白，推测为一种分泌型肽激素的前体。应用于拟南芥原位组织的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）确定了一种修饰的12氨基酸肽（MCLV3）的结构，该肽源自CLV3序列中的一个保守基序。合成的MCLV3随着细胞分化为其他器官而诱导茎尖和根尖分生组织消耗，表现出过表达CLV3的转基因植物的典型表型。这些结果表明CLV3的功能肽是MCLV3。
