Bright S A, Bitman J, Capuco A V, Wood D L, Miller R H
Livestock and Poultry Science Institute, US Department of Agriculture, Beltsville, MD 20705.
J Dairy Sci. 1990 Jan;73(1):98-106. doi: 10.3168/jds.S0022-0302(90)78651-1.
Methods for collecting keratin from the teat canal were examined to select a procedure to obtain representative samples for lipid analysis. Data obtained by solvent extraction of excised teats were compared with those obtained by scraping keratin from dissected teats of lactating and dry cows. Solvent extraction with petroleum ether or 2:1 chloroform-methanol yielded similar dry weights of material. However, both solvents removed large amounts of material other than keratin from the teat canal. The lipid class and fatty acid profiles of the material extracted by solvent flushing were not similar to profiles obtained by scraping keratin from the teat canal. A metal tapestry needle was suitable for collection of keratin from the teat canal of living cows. About 78% of the keratin present in the teat was collected with the needle. Lipid composition of keratin collected with the needle was the same as in keratin scraped from excised teats. The tapestry needle was suitable as a tool for collecting repeatable, representative samples of keratin for analysis from single teat canals of living cows.
研究了从乳头管收集角蛋白的方法，以选择一种获取用于脂质分析的代表性样本的程序。将通过溶剂萃取切除乳头获得的数据与通过刮取泌乳期和干奶牛解剖乳头的角蛋白获得的数据进行比较。用石油醚或2:1氯仿 - 甲醇进行溶剂萃取得到的物质干重相似。然而，两种溶剂都从乳头管中除去了大量角蛋白以外的物质。通过溶剂冲洗提取的物质的脂质类别和脂肪酸谱与通过刮取乳头管角蛋白获得的谱不相似。金属织锦针适用于从活体奶牛的乳头管收集角蛋白。用该针收集了乳头中约78%的角蛋白。用该针收集的角蛋白的脂质组成与从切除乳头刮取的角蛋白相同。织锦针适合作为从活体奶牛单个乳头管收集可重复、代表性角蛋白样本进行分析的工具。
