Sun Hai-Yan, Ge Xiang-Yang, Zhang Wei-Guo
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, JiangSu Province, P.R. China.
Biotechnol Lett. 2006 Nov;28(21):1719-23. doi: 10.1007/s10529-006-9151-2. Epub 2006 Aug 11.
A newly isolated strain, Penicillium sp. S-22, was used to produce an enzyme that hydrolyses raw yam starch [raw yam starch digesting enzyme (RYSDE)]. The enzyme activity and overall enzyme productivity were respectively 16 U/ml and 0.19 U/ml h in the batch culture. The enzyme activity increased to 85 U/ml by feeding of partially hydrolyzed raw yam starch. When a mixture containing partially hydrolyzed raw yam starch and peptone was fed by a pH-stat strategy, the enzyme activity reached 366 U/ml, 23-fold of that obtained in the batch culture, and the overall productivity reached 3.4 U/ml h, which was 18-fold of that in the batch culture.
一种新分离的菌株，青霉属S-22，被用于生产一种可水解生山药淀粉的酶[生山药淀粉消化酶（RYSDE）]。在分批培养中，该酶的活性和总酶产量分别为16 U/ml和0.19 U/ml·h。通过添加部分水解的生山药淀粉，酶活性提高到了85 U/ml。当采用pH值恒控策略添加含有部分水解的生山药淀粉和蛋白胨的混合物时，酶活性达到366 U/ml，是分批培养中所得酶活性的23倍，总生产率达到3.4 U/ml·h，是分批培养中的18倍。
