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人MRP/Th RNA及其核基因的特性：全长MRP/Th RNA组装成核糖核蛋白时是一种活性核糖核酸内切酶。

Characterization of human MRP/Th RNA and its nuclear gene: full length MRP/Th RNA is an active endoribonuclease when assembled as an RNP.

作者信息

Topper J N, Clayton D A

机构信息

Department of Developmental Biology, Stanford University School of Medicine, CA 94305-5427.

出版信息

Nucleic Acids Res. 1990 Feb 25;18(4):793-9. doi: 10.1093/nar/18.4.793.

DOI:10.1093/nar/18.4.793
PMID:1690392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330329/
Abstract

Vertebrate cells contain a site-specific endoribonuclease (RNase MRP) that cleaves mitochondrial RNA transcribed from the origin of leading-strand mitochondrial DNA replication. This report presents the characterization of the human enzyme and its essential RNA component. Human RNase MRP is a ribonucleoprotein with a nucleus-encoded RNA of 265 nucleotides. As expected, the single-copy RNA coding region is homologous (84%) to the corresponding mouse gene; surprisingly, at least 700 nucleotides of the immediate 5'-flanking region are conserved. The 265-nucleotide MRP RNA and an MRP RNA cleavage product representing the 3'-terminal 108 nucleotides exist in nuclear and mitochondrial RNA isolates; the larger MRP RNA is present in greatest abundance in the nucleus. The putative processing site within the 265-nucleotide MRP RNA is offset from that of mouse MRP RNA, but in each case cleavage is precise and occurs at the sequence ANCCCGC. Oligonucleotide-mediated inhibition experiments reveal that both the 5' and 3' portions of the MRP RNA are involved in cleavage by RNase MRP; this implies that full length MRP RNA complexed with proteins is an active species in vertebrate cells.

摘要

脊椎动物细胞含有一种位点特异性核糖核酸内切酶（RNase MRP），它能切割从前导链线粒体DNA复制起点转录而来的线粒体RNA。本报告介绍了人类该酶及其必需RNA成分的特性。人类RNase MRP是一种核糖核蛋白，其核编码RNA为265个核苷酸。正如预期的那样，单拷贝RNA编码区与相应的小鼠基因同源（84%）；令人惊讶的是，紧邻的5'侧翼区至少700个核苷酸是保守的。265个核苷酸的MRP RNA和代表3'末端108个核苷酸的MRP RNA切割产物存在于核RNA和线粒体RNA分离物中；较大的MRP RNA在细胞核中含量最丰富。265个核苷酸的MRP RNA中的推定加工位点与小鼠MRP RNA的不同，但在每种情况下切割都是精确的，且发生在序列ANCCCGC处。寡核苷酸介导的抑制实验表明，MRP RNA的5'和3'部分都参与了RNase MRP的切割；这意味着与蛋白质复合的全长MRP RNA在脊椎动物细胞中是一种活性物质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/27cce06a35d1/nar00188-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/8f0691fbebf6/nar00188-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/623bd52f127f/nar00188-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/cb78bf9f66eb/nar00188-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/27cce06a35d1/nar00188-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/8f0691fbebf6/nar00188-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/623bd52f127f/nar00188-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/cb78bf9f66eb/nar00188-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e5/330329/27cce06a35d1/nar00188-0101-a.jpg

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