Aranda Cristina, Colón Maritrini, Ishida Cecilia, Riego Lina, Deluna Alexander, Valenzuela Lourdes, Herrera Jorge, González Alicia
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510México D.F., Mexico.
Biochem Biophys Res Commun. 2006 Sep 29;348(3):989-96. doi: 10.1016/j.bbrc.2006.07.154. Epub 2006 Aug 1.
Analysis of the UGA3-GLT1 bidirectional promoter has indicated that its transcriptional activation is determined by the combined action of Gcn4p and Gln3p, and that its bidirectional character is influenced by chromatin organization, through the action of an Abf1p binding site and a polydAdTtract. Results presented in this paper show that lack of Gcn5p impairs histone acetylation and nucleosomal organization of the UGA3-GLT1 promoter, resulting in an asymmetrical transcriptional activation response of UGA3 and GLT1. The phenotype displayed by a double mutant impaired in GCN5 and in the Abf1p binding site indicates that the combined action of these two elements determines the bidirectional capacity of the UGA3-GLT1 intergenic region.
对UGA3 - GLT1双向启动子的分析表明，其转录激活由Gcn4p和Gln3p的联合作用决定，并且其双向特性受染色质组织的影响，通过Abf1p结合位点和多聚dAdT序列的作用。本文给出的结果表明，Gcn5p的缺失会损害UGA3 - GLT1启动子的组蛋白乙酰化和核小体组织，导致UGA3和GLT1的不对称转录激活反应。在GCN5和Abf1p结合位点均有缺陷的双突变体所表现出的表型表明，这两个元件的联合作用决定了UGA3 - GLT1基因间区域的双向能力。
