肾型心钠素受体的结合特性与功能对去氧皮质酮-盐诱导的大鼠高血压具有抗性。

Renal atrial natriuretic peptide receptors binding properties and function are resistant to DOCA-salt-induced hypertension in rats.

作者信息

Li Xiaohong, Woodard Geoffrey E, Brown John, Rosado Juan A

机构信息

National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Rm 8C-208, 10 Center Drive, MSC 1752, Bethesda, MD 20892-1752, USA.

出版信息

Regul Pept. 2006 Dec 10;137(3):114-20. doi: 10.1016/j.regpep.2006.06.003. Epub 2006 Aug 10.

DOI:10.1016/j.regpep.2006.06.003

PMID:16904201

Abstract

Atrial natriuretic peptide receptor types A (NPR-A) and C (NPR-C) binding properties and functional characteristics in renal glomeruli have been investigated in deoxycorticosterone acetate (DOCA)-treated hypertensive Wistar-Kyoto (WKY) rats and their respective controls. We found that DOCA administration had no significant effect on the maximum binding capacity or the affinity of renal NPR-A and NPR-C. NPR-C is involved in the regulation of cAMP production. Our results indicate that the cAMP production by NPR-C is not altered in DOCA-induced hypertension, since ANP(1-28), CNP(1-22) and C-ANP, which specifically bind to NPR-C, show a similar inhibitory effect on cAMP production stimulated by the physiological agonist histamine in glomeruli from DOCA-treated rats and controls. Finally, we have found that DOCA-induced hypertension does not modify NPR-A or NPR-C expression in rat glomerular membranes. These findings indicate that NPR-A and NPR-C binding properties and NPR-C-mediated inhibition of cAMP generation remain unaltered in DOCA-treated rats.

摘要

在接受醋酸脱氧皮质酮（DOCA）治疗的高血压Wistar-Kyoto（WKY）大鼠及其各自的对照组中，研究了肾小球中A型心房利钠肽受体（NPR-A）和C型心房利钠肽受体（NPR-C）的结合特性和功能特征。我们发现，给予DOCA对肾脏NPR-A和NPR-C的最大结合容量或亲和力没有显著影响。NPR-C参与cAMP生成的调节。我们的结果表明，在DOCA诱导的高血压中，NPR-C介导的cAMP生成没有改变，因为特异性结合NPR-C的ANP(1-28)、CNP(1-22)和C-ANP，对DOCA处理的大鼠和对照组肾小球中由生理激动剂组胺刺激的cAMP生成显示出相似的抑制作用。最后，我们发现DOCA诱导的高血压不会改变大鼠肾小球膜中NPR-A或NPR-C的表达。这些发现表明，在DOCA处理的大鼠中，NPR-A和NPR-C的结合特性以及NPR-C介导的对cAMP生成的抑制作用保持不变。