La Rosa F G, Talmage D W
Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80262.
Transplantation. 1990 Mar;49(3):605-9. doi: 10.1097/00007890-199003000-00024.
H-2d thyroids cultured in oxygen at normal atmospheric pressure (suboptimal culture) were grafted into H-2b mice. Some of these tissues were cultured with recombinant mouse gamma-interferon (rIFN), and they expressed high levels of major histocompatibility complex antigens before grafting. Three weeks later, no difference in the rate of rejection of MHC-induced grafts was observed as compared with uninduced tissues (50% of each group). Fresh (uncultured) grafts were uniformly rejected in less than 2 weeks. Also, H-2d thyroids, freed of donor leukocytes by preculture in hyperbaric oxygen and more than 1 year parking in normal H-2b recipients, were incubated with and without rIFN, and then grafted into normal H-2b mice; 100% acceptance was observed in both groups regardless of the expression of allo-MHC molecules on thyroid cells. In another set of experiments, using grafts with a single antigenic difference at the MHC locus (bm1 into B6), graft rejection was observed only when the recipients were immunized with donor spleen cells and fresh tissues were implanted. In the same immune recipients, cultured and MHC-induced thyroids grafted in the opposite kidney were, in general, not rejected. These results demonstrated that the expression of allo-MHC molecules on graft cells was not by itself sufficient to engender tissue immunogenicity. This supports our previous hypothesis that the main effect of tissue culture is the inactivation of passenger leukocytes. MHC antigens appear to be immunogenic only when properly presented by these cells.
在正常大气压下于氧气中培养的H-2d甲状腺（次优培养）被移植到H-2b小鼠体内。其中一些组织用重组小鼠γ干扰素（rIFN）培养，并且在移植前它们表达高水平的主要组织相容性复合体抗原。三周后，与未诱导的组织相比，MHC诱导的移植物的排斥率没有差异（每组50%）。新鲜（未培养）的移植物在不到2周内均被排斥。此外，通过在高压氧中预培养并在正常H-2b受体中停放超过1年以去除供体白细胞的H-2d甲状腺，在有或没有rIFN的情况下进行孵育，然后移植到正常H-2b小鼠体内；无论甲状腺细胞上同种异体MHC分子的表达如何，两组均观察到100%的接受率。在另一组实验中，使用在MHC位点有单一抗原差异的移植物（bm1到B6），仅当受体用供体脾细胞免疫并植入新鲜组织时才观察到移植物排斥。在相同的免疫受体中，移植到对侧肾脏的培养的和MHC诱导的甲状腺通常不会被排斥。这些结果表明，移植细胞上同种异体MHC分子的表达本身不足以产生组织免疫原性。这支持了我们之前的假设，即组织培养的主要作用是使过客白细胞失活。MHC抗原似乎只有在这些细胞正确呈递时才具有免疫原性。
