Sustar Maja, Hawlina Marko, Brecelj Jelka
Eye Clinic, University Medical Centre, Zaloska 29/a, 1000, Ljubljana, Slovenia.
Doc Ophthalmol. 2006 Jul;113(1):43-52. doi: 10.1007/s10633-006-9013-1. Epub 2006 Aug 12.
The aim of this study was to explore the effect of stimulus duration, stimulus intensity, stimulus wavelength and background luminance on the amplitude and waveform of the ON- (b-wave) and OFF- (d-wave) response of the photopic ERG. The following parameters were used in this study: stimulus duration from 5 to 200 ms, the flash intensities from 0.4 to 2.1 log cd s/m2, the background luminance from 20 to 50 cd/m2 and stimulus wavelengths 460, 508 and 667 nm. Prolonging the stimulus duration from 75 to 200 ms did not influence the amplitudes of a-, b- and d-waves significantly. Higher stimulus intensities (1.9 log cd s/m2) broadened the b-wave and increased variability of its implicit time. With a brighter background (50 cd/m2) the b-wave did not broaden and its optimal amplitude was preserved. Stimuli of longer wavelengths (667 nm) significantly reduced the d-wave amplitude. Our results suggest that high intensities and longer wavelengths (red) may not be suitable stimulus parameters in the routine clinical ON- and OFF-response recording.
本研究的目的是探讨刺激持续时间、刺激强度、刺激波长和背景亮度对明视视网膜电图ON（b波）和OFF（d波）反应的振幅和波形的影响。本研究使用了以下参数：刺激持续时间为5至200毫秒，闪光强度为0.4至2.1 log cd s/m²，背景亮度为20至50 cd/m²，刺激波长为460、508和667纳米。将刺激持续时间从75毫秒延长至200毫秒对a波、b波和d波的振幅没有显著影响。较高的刺激强度（1.9 log cd s/m²）使b波变宽并增加了其潜伏时的变异性。在较亮的背景（50 cd/m²）下，b波没有变宽且保持了其最佳振幅。较长波长（667纳米）的刺激显著降低了d波振幅。我们的结果表明，在常规临床ON和OFF反应记录中，高强度和较长波长（红色）可能不是合适的刺激参数。
