Williams C A, Allen-Hoffmann B L
Environmental Toxicology Center, University of Wisconsin, Madison 53706.
J Biol Chem. 1990 Apr 15;265(11):6467-72.
Transforming growth factor-beta (TGF-beta 1) suppresses cortisol production when added to cultured bovine adrenocortical (BAC) cells while concomitantly increasing fibronectin synthesis and assembly into extracellular fibrils. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gelatin-Sepharose-treated media from BAC cells demonstrated a 2-fold stimulation of fibronectin production by TGF-beta 1 in both the presence and absence of serum. Indirect immunofluorescence studies revealed that TGF-beta 1 caused a striking increase in the fibronectin content of BAC extracellular matrix. TGF-beta 1 caused a 4-fold increase in deoxycholate-insoluble fibronectin after 12 h and a 7-fold increase after 24 h over that of control BAC cultures. Northern hybridization analyses indicated that TGF-beta 1 stimulated levels of fibronectin poly(A)+ RNA 2.3-fold. We found that cultured BAC cells express TGF-beta 1 mRNA, suggesting a possible autocrine role for TGF-beta 1 in the adrenal.
转化生长因子-β（TGF-β1）添加到培养的牛肾上腺皮质（BAC）细胞中时，可抑制皮质醇的产生，同时增加纤连蛋白的合成并组装到细胞外纤维中。对BAC细胞经明胶-琼脂糖凝胶处理的培养基进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，无论有无血清，TGF-β1均可使纤连蛋白的产生增加2倍。间接免疫荧光研究表明，TGF-β1可使BAC细胞外基质中的纤连蛋白含量显著增加。与对照BAC培养物相比，TGF-β1在12小时后使脱氧胆酸盐不溶性纤连蛋白增加4倍，24小时后增加7倍。Northern杂交分析表明，TGF-β1可使纤连蛋白多聚腺苷酸+RNA水平提高2.3倍。我们发现培养的BAC细胞表达TGF-β1 mRNA，提示TGF-β1在肾上腺中可能具有自分泌作用。
