禾谷梨孢菌乳清酸核苷-5'-单磷酸脱羧酶基因的克隆、序列分析及异源表达

Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5'-monophosphate decarboxylase gene.

作者信息

De Maeseneire Sofie L, De Groeve Manu R M, Dauvrin Thierry, De Mey Marjan, Soetaert Wim, Vandamme Erick J M C

机构信息

Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Belgium.

出版信息

FEMS Microbiol Lett. 2006 Aug;261(2):262-71. doi: 10.1111/j.1574-6968.2006.00373.x.

DOI:10.1111/j.1574-6968.2006.00373.x

PMID:16907730

Abstract

A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide sequence and the amino acid sequence were compared with fungal OMPD sequences. They show the highest similarity to OMPD genes and enzymes of Aspergillus sp., Penicillium sp. and Cladosporium fulvum. The functionality of the gene as a selection marker was proven by complementation of the uracil auxotrophy of Aspergillus nidulans FGSC A722.

摘要

从禾谷梨孢菌基因组中分离出一段2918 bp的序列，该序列编码乳清苷-5'-单磷酸脱羧酶（OMPD）。对该序列进行分析后发现，它是第一个来自粪壳菌纲且含有内含子的OMPD基因。该基因编码一种由282个氨基酸组成的酶。将核苷酸序列和氨基酸序列与真菌OMPD序列进行比较，结果显示它们与曲霉属、青霉属和番茄叶霉的OMPD基因及酶具有最高的相似性。通过对构巢曲霉FGSC A722的尿嘧啶营养缺陷型进行互补，证明了该基因作为选择标记的功能。