产朊假丝酵母乳清苷-5'-磷酸脱羧酶基因（URA3）的分离与序列分析。与OMP脱羧酶基因家族的比较。
Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family.
作者信息
Rodríguez L, Chávez F P, González M E, Basabe L, Rivero T
机构信息
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
出版信息
Yeast. 1998 Nov;14(15):1399-406. doi: 10.1002/(SICI)1097-0061(199811)14:15<1399::AID-YEA324>3.0.CO;2-D.
The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.
通过在大肠杆菌pyrF突变体中进行互补，分离出了产朊假丝酵母编码乳清苷-5'-磷酸脱羧酶的URA3基因。推导的氨基酸序列与其他酵母和真菌物种的Ura3蛋白高度相似。展示了对乳清苷-5'-磷酸脱羧酶家族的广泛分析。产朊假丝酵母的URA3基因能够在功能上互补酿酒酵母的ura3突变。
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