含羧基侧链的锌金属化腺嘌呤聚合物对磷酸二酯的修饰

Phosphodiester modification by zinc metalated adenine polymer with carboxyl pendants.

作者信息

Gupta Yogita, Mathur G N, Parvez Masood, Verma Sandeep

机构信息

Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur 208016, India.

出版信息

Bioorg Med Chem Lett. 2006 Oct 15;16(20):5364-7. doi: 10.1016/j.bmcl.2006.07.077.

DOI:10.1016/j.bmcl.2006.07.077

PMID:16908147

Abstract

This report describes a novel carboxyl pendant containing adenylated polymeric template, its metalation with Zn (II), and manifestation of catalytic activity for the hydrolysis of model phosphodiester, bis(p-nitrophenyl) phosphate (bNPP), and plasmid cleavage. Observation of a bell-shaped pH-K(obs) profile suggested influence of pH variation over hydrolysis rate. This metalated polymer also afforded facile relaxation of pBR322 supercoiled DNA, with an interesting reusability feature intricately associated with heterogeneous catalysis.

摘要

本报告描述了一种含腺苷酸化聚合物模板的新型羧基侧基、其与锌（II）的金属化以及对模型磷酸二酯双（对硝基苯基）磷酸酯（bNPP）水解和质粒切割的催化活性表现。钟形pH-K(obs)曲线的观察表明pH变化对水解速率有影响。这种金属化聚合物还能使pBR322超螺旋DNA轻松松弛，具有与多相催化复杂相关的有趣的可重复使用特性。