Olofsson R, Oltean M, Olausson M
Department of Transplantation and Liver Surgery, Sahlgrenska University Hospital, 413-45 Göteborg, Sweden.
Transplant Proc. 2006 Jul-Aug;38(6):1789-91. doi: 10.1016/j.transproceed.2006.05.046.
Comparison between the extensive information on intestinal preservation injury in the rat is challenging since various preservation solutions, technical details, and grading systems have been used. This study investigates if strain represents another relevant variable for preservation injury outcome.
Grafts from Piebald-Viral-Glaxo (PVG), Lewis, Brown Norway (BN), Wistar, and Sprague-Dawley (SD) male rats (n = 8/strain) were used. Grafts were perfused and stored at 4 degrees C in University of Wisconsin (UW) solution (with or without luminal preservation). Intestinal histology was evaluated at 8, 16, and 24 hours of preservation using the Park score. Lactate, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and glucose were measured in the preservation solution.
Grafts from PVG and Lewis showed significantly lower injury scores compared to BN, Wistar, and SD at 8 and 16 hours, a difference that disappeared at 24 hours of preservation. Luminal preservation significantly reduced the injury score for all strains, except SD, at 8 and at 16 hours, a difference that disappeared at 24 hours. Biochemical analyses of LDH and lactate levels show a similar pattern both between different strains and the effect of luminal preservation, although not statistically significant.
Different rat strains seem to have different susceptibility to intestinal preservation injury under identical conditions. These results indicate that PVG and Lewis are more resistant to preservation injury than Wistar, BN, or SD. The beneficial effect of luminal preservation with UW solution is limited after 8 to 16 hours.
由于使用了各种保存溶液、技术细节和分级系统，对大鼠肠道保存损伤的大量信息进行比较具有挑战性。本研究调查品系是否代表保存损伤结果的另一个相关变量。
使用来自花斑-病毒-葛兰素（PVG）、刘易斯、棕色挪威（BN）、Wistar和斯普拉格-道利（SD）雄性大鼠的移植物（每个品系n = 8）。移植物在威斯康星大学（UW）溶液中于4℃灌注并储存（有或无肠腔保存）。使用帕克评分在保存8、16和24小时时评估肠道组织学。在保存溶液中测量乳酸、乳酸脱氢酶（LDH）、碱性磷酸酶（ALP）和葡萄糖。
与BN、Wistar和SD相比，PVG和刘易斯的移植物在8和16小时时损伤评分显著更低，这种差异在保存24小时时消失。肠腔保存在8和16小时时显著降低了所有品系（除SD外）的损伤评分，这种差异在24小时时消失。LDH和乳酸水平的生化分析在不同品系之间以及肠腔保存的影响方面显示出类似模式，尽管无统计学意义。
在相同条件下，不同大鼠品系对肠道保存损伤似乎具有不同的易感性。这些结果表明，PVG和刘易斯比Wistar、BN或SD对保存损伤更具抵抗力。使用UW溶液进行肠腔保存的有益效果在8至16小时后有限。
