Omae Hiromichi, Mochizuki Yu, Yokoya Shin, Adachi Nobuo, Ochi Mitsuo
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8551, Japan.
Int Orthop. 2007 Jun;31(3):353-8. doi: 10.1007/s00264-006-0194-8. Epub 2006 Aug 15.
Tendon attachment to interconnected porous calcium hydroxyapatite ceramics (IP-CHA) with cultured bone marrow stromal cells (BMSC) was analysed. The purpose of this study was to evaluate whether BMSC in IP-CHA could augment the tendon attachment to IP-CHA histologically and biomechanically. Eighteen Japanese white rabbits were used. Cultured BMSCs were subcultured in IP-CHA. The grafted tendon and IP-CHA with BMSC complex were implanted in a bone defect of the knee [BMSC(+) group]. In the contralateral knee, a tendon and IP-CHA without BMSC complex were implanted [BMSC(-) group]. Histological findings of the interface between the tendon and IP-CHA were similar in the two groups 3 weeks after the operation. However, 6 weeks after the operation, more abundant bone formation around the tendon was observed in the BMSC(+) group. The direct apposition of the tendon to bone in pores and collagen fibre continuity between the tendon and fibrous tissue in pores were observed. In biomechanical evaluation, the maximum pull-out load of the tendon from the IP-CHA in the BMSC(+) group was significantly higher than that in the BMSC(-) group 6 weeks after the operation. BMSCs cultured in IP-CHA could augment tendon attachment to IP-CHA.
分析了肌腱与互连多孔羟基磷灰石陶瓷（IP-CHA）以及培养的骨髓基质细胞（BMSC）的附着情况。本研究的目的是评估IP-CHA中的BMSC是否能在组织学和生物力学方面增强肌腱与IP-CHA的附着。使用了18只日本白兔。将培养的BMSC在IP-CHA中传代培养。将移植的肌腱与含有BMSC复合物的IP-CHA植入膝关节骨缺损处[BMSC（+）组]。在对侧膝关节，植入不含BMSC复合物的肌腱和IP-CHA[BMSC（-）组]。术后3周，两组肌腱与IP-CHA界面的组织学表现相似。然而，术后6周，BMSC（+）组肌腱周围观察到更丰富的骨形成。观察到肌腱在孔隙中与骨的直接贴合以及肌腱与孔隙中纤维组织之间的胶原纤维连续性。在生物力学评估中，术后6周，BMSC（+）组中肌腱从IP-CHA的最大拔出负荷显著高于BMSC（-）组。在IP-CHA中培养的BMSC可增强肌腱与IP-CHA的附着。
