Bondarenko O I, Sahach V F
Fiziol Zh (1994). 2006;52(3):34-41.
The role of Na pump in the regulation of the membrane potential of endothelial cells of rat isolated aorta at rest and during stimulation by acetylcholine was investigated. Inhibition of Na pump by 500 mcM ouabain and potassium-free solution depolarized endothelial cells by 11 mV. Na pump reactivation caused by potassium restoration hyperpolarized endothelium by 18 mV, this hyperpolarization was sensitive to 500 mcM ouabain. Potassium-free solution and ouabain attenuated the duration of the acetylcholine-evoked hyperpolarization. The results presented demonstrate that the sustained hyperpolarization of endothelial cells to acetylcholine in rat aorta may be partially mediated by Na pump stimulation.
研究了钠泵在静息状态下以及乙酰胆碱刺激时对大鼠离体主动脉内皮细胞膜电位调节中的作用。500 μM哇巴因和无钾溶液对钠泵的抑制作用使内皮细胞去极化11 mV。钾离子恢复引起的钠泵再激活使内皮细胞超极化18 mV，这种超极化对500 μM哇巴因敏感。无钾溶液和哇巴因缩短了乙酰胆碱诱发的超极化持续时间。所呈现的结果表明，大鼠主动脉内皮细胞对乙酰胆碱的持续性超极化可能部分由钠泵刺激介导。
