Roy P, Noad R
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Curr Top Microbiol Immunol. 2006;309:87-116. doi: 10.1007/3-540-30773-7_4.
Like other members of the Reoviridae, bluetongue virus faces the same constraints on structure and assembly that are imposed by a large dsRNA genome. However, since it is arthropod-transmitted, BTV must have assembly pathways that are sufficiently flexible to allow it to replicate in evolutionarily distant hosts. With this background, it is hardly surprising that BTV interacts with highly conserved cellular pathways during morphogenesis and trafficking. Indeed, recent studies have revealed striking parallels between the pathways involved in the entry and egress of nonenveloped BTV and those used by enveloped viruses. In addition, recent studies with the protein that is the major component of the BTV viroplasm have revealed how the assembly and, as importantly, the disassembly of this structure may be achieved. This is a first step towards resolving the interactions that occur in these virus 'assembly factories'. Overall, this review demonstrates that the integration of structural, biochemical and molecular data is necessary to fully understand the assembly and replication of this complex RNA virus.
与呼肠孤病毒科的其他成员一样，蓝舌病毒在结构和组装方面面临着由大型双链RNA基因组所带来的相同限制。然而，由于它是通过节肢动物传播的，蓝舌病毒必须拥有足够灵活的组装途径，以使其能够在进化上距离遥远的宿主中进行复制。基于这样的背景，蓝舌病毒在形态发生和运输过程中与高度保守的细胞途径相互作用也就不足为奇了。事实上，最近的研究揭示了无包膜蓝舌病毒的进入和释放途径与包膜病毒所使用的途径之间存在惊人的相似之处。此外，最近对作为蓝舌病毒病毒质主要成分的蛋白质的研究揭示了这种结构的组装以及同样重要的拆卸是如何实现的。这是解决在这些病毒“组装工厂”中发生的相互作用的第一步。总体而言，这篇综述表明，整合结构、生化和分子数据对于全面理解这种复杂RNA病毒的组装和复制是必要的。
