Drobnic-Kosorok M, Kopitar M, Babnik J, Jerala R, Turk V
Odsek za biokemijo, Institut J. Stefan, Univerza E. Kardelja, Ljubljana, Jugoslavija.
Biol Chem Hoppe Seyler. 1990 Jan;371(1):57-61. doi: 10.1515/bchm3.1990.371.1.57.
A new intracellular inhibitor of plasmin and trypsin was isolated from porcine leukocytes by ion exchange chromatography and affinity chromatography. In dodecyl sulphate gel electrophoresis a single protein band with an apparent molecular mass of 15 kDa was found under reducing conditions. On isoelectric focusing three protein bands with isoelectric points between pH 4.0 and 4.5 were found. The association rate constants and the inhibition constants were determined for porcine plasmin and bovine trypsin. The inhibitor shows no immunologic cross-reactivity with any of the tested leukocyte inhibitors. On the basis of its N-terminal amino-acid sequence a great degree of similarity to Kunitz-type inhibitors was observed.
通过离子交换色谱法和亲和色谱法从猪白细胞中分离出一种新的纤溶酶和胰蛋白酶细胞内抑制剂。在十二烷基硫酸盐凝胶电泳中，在还原条件下发现一条表观分子量为15 kDa的单一蛋白条带。在等电聚焦时，发现了三条等电点在pH 4.0至4.5之间的蛋白条带。测定了该抑制剂对猪纤溶酶和牛胰蛋白酶的结合速率常数和抑制常数。该抑制剂与任何测试的白细胞抑制剂均无免疫交叉反应。根据其N端氨基酸序列，发现它与库尼茨型抑制剂有很大程度的相似性。
