Klotz Judith, Bryant Patricia, Wilcox Homer B, Dillon Mary, Wolf Bruce, Fagliano Jerald
School of Public Health, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, USA.
Paediatr Perinat Epidemiol. 2006 Sep;20(5):449-52. doi: 10.1111/j.1365-3016.2006.00749.x.
We have demonstrated the feasibility of linking newborn blood spots, population-based cancer incidence data and birth certificate data. Incident cases of acute lymphocytic leukaemia and population-based controls were ascertained. We retrieved dried blood spot specimens, isolated and amplified DNA, and assayed the cancer susceptibility genes GSTT1 and GSTM1. The double null genotype was over-represented in the cases, consistent with previous reports based on other epidemiological methods. The design avoids issues of participation bias by cases and controls and can be used to investigate interactions of susceptibility genes and xenobiotics in semi-ecological studies. It can be useful for generating or testing hypotheses on associations of other paediatric illness and environmental contaminants.
我们已经证明了将新生儿血斑、基于人群的癌症发病率数据和出生证明数据相联系的可行性。确定了急性淋巴细胞白血病的发病病例和基于人群的对照。我们检索了干血斑标本，分离并扩增了DNA，并检测了癌症易感基因GSTT1和GSTM1。双无效基因型在病例中过度表达，这与基于其他流行病学方法的先前报告一致。该设计避免了病例和对照的参与偏倚问题，可用于半生态学研究中调查易感基因与外源性物质的相互作用。它对于生成或检验关于其他儿科疾病与环境污染物关联的假设可能有用。
