Liu Ying, Chen Chen, Wu Wei-Kang, Duan Xin-Feng
Department of Pathophysiology, Guangdong Pharmacological College, Guangzhou 510224, China.
Zhong Yao Cai. 2006 Apr;29(4):345-8.
The present study was designed to determine the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND).
Sprage-Dawleytrats were divide into four groups: control, sham, I/R and SND groups. Left anterior descending coronary artery (LAD) of the rats in I/R group were occluded for h and reperfused for h. SND group rates were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days and the last treatment was pretreated 24h before the index occlusion. Cell apoptosis was measured by flow cytometry, mRNA level of bcl-xl/bcl-xs was detected by RT-PCR and apoptosis inducing factor (AIF) was detected by western blotting.
SND pretreatment for 24 hours could decrease cell apoptosis, increase bcl-xl-bcl-xs mRNA ratio and decrease release of AIF from mitochondria.
The delayed preconditioning induced by SND can decrease myocardial cell apoptosis. The mechanism is related to mitochondria protection.
