聚合酶链式反应简介。

Introduction to the polymerase chain reaction.

作者信息

Lo Y M Dennis, Chan K C Allen

机构信息

Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR.

出版信息

Methods Mol Biol. 2006;336:1-10. doi: 10.1385/1-59745-074-X:1.

DOI:10.1385/1-59745-074-X:1

PMID:16916248

Abstract

The polymerase chain reaction (PCR) is an in vitro method for the amplification of DNA. Since the introduction of the PCR in 1985, it has become an indispensable technique for many applications in scientific research and clinical and forensic investigations. In this chapter, the principle and setup of PCR, as well as the methods for analyzing PCR products, will be discussed.

摘要

聚合酶链反应（PCR）是一种用于DNA扩增的体外方法。自1985年PCR被引入以来，它已成为科学研究、临床及法医调查中许多应用不可或缺的技术。在本章中，将讨论PCR的原理、设置以及分析PCR产物的方法。