Vennix P P, Kuijpers W, Tonnaer E L, Peters T A, Ramaekers F C
Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
Arch Otolaryngol Head Neck Surg. 1990 May;116(5):560-5. doi: 10.1001/archotol.1990.01870050060007.
The expression of cytokeratins varies with the type of epithelium, the state of differentiation, and pathological conditions. In this study, the differential expression of cytokeratins in external meatal skin and middle ear epithelium was used for a pathogenetic study of cholesteatoma lesions and infection-induced epidermoid formations in the middle ear of the rat. Immunocytochemistry generally revealed an epidermal-type cytokeratin profile in the cholesteatoma matrix, except for the focal expression of nonepidermal cytokeratins at the invasion front. Comparable observations were made in the middle ear of the rat after an infection-induced invasion of epidermal cells from the meatal skin. An infection-induced-cornifying metaplastic lesion of the middle ear epithelium revealed nonepidermal cytokeratin expression. The results of this combined study suggested that the cholesteatoma specimens studied had an epidermal origin. The expression of nonepidermal cytokeratins was considered to result from a state of hyperproliferation rather than from metaplasia.
细胞角蛋白的表达随上皮类型、分化状态及病理状况而变化。在本研究中，利用外耳道皮肤和中耳上皮中细胞角蛋白的差异表达，对大鼠中耳胆脂瘤病变及感染诱导的表皮样形成进行发病机制研究。免疫细胞化学一般显示胆脂瘤基质中存在表皮型细胞角蛋白谱，但在侵袭前沿有非表皮细胞角蛋白的局灶性表达。在大鼠中耳，当外耳道皮肤的表皮细胞因感染而侵入时，也观察到了类似的现象。中耳上皮的感染诱导角化化生病变显示有非表皮细胞角蛋白表达。这项联合研究的结果表明，所研究的胆脂瘤标本起源于表皮。非表皮细胞角蛋白的表达被认为是过度增殖状态而非化生所致。
